WB
H M R
Endogenous
100
Rabbit IgG
#Q9UQ16
26052
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser783 of human dynamin 3 protein.
Background
Dynamin is a family of large GTPases that has been implicated in the formation of vesicles of both endocytotic and secretory processes (1). Dynamin plays an important role in the internalization of cell surface receptors, a process that attenuates the response to extracellular signals. There are three isoforms of dynamin. Dynamin 1 and 3 expression is enriched in the brain, while dynamin 2 expression is ubiquitous. All three isoforms mediate membrane fission during clathrin-mediated endocytosis. Dynamin 2 is also suggested to be involved in non-clathrin-mediated endocytosis and cytoskeletal interactions (2-4). Dynamin 3 is suggested to regulate AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptor recycling through its interaction with the postsynaptic density scaffolding proteins Homer and Shank (5-7). In addition to the role they play in neurotransmission, dynamin family members are considered therapeutic targets in cancer. Dysregulation of endocytosis in cancer leads to dysfunction in cell signaling, survival, and proliferation. Dynamin 1 and 2 have been shown to promote tumorigenesis, while dynamin 3 is a suggested tumor suppressor (8-12).
- McNiven, M.A. et al. (2000) Trends Biochem Sci 25, 115-20.
- Raimondi, A. et al. (2011) Neuron 70, 1100-14.
- Calabrese, B. and Halpain, S. (2015) Mol Cell Neurosci 68, 36-45.
- Trochet, D. and Bitoun, M. (2021) J Exp Clin Cancer Res 40, 238.
- Lu, J. et al. (2007) Neuron 55, 874-89.
- Gray, N.W. et al. (2003) Curr Biol 13, 510-5.
- Nakano-Kobayashi, A. et al. (2014) J Neurosci 34, 8665-71.
- Inokawa, Y. et al. (2013) Onco Targets Ther 6, 1417-24.
- Fa, J. (2021) Oncol Lett 22, 524.
- Lu, Q. et al. (2021) Front Cell Dev Biol 9, 641403.
- Gu, C. et al. (2017) Oncol Lett 13, 4776-4784.
- Meng, J. (2017) Oncotarget 8, 41701-41716.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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