WB, ChIP
H
Endogenous
70
Rabbit
#Q01094
1869
Product Information
Product Usage Information
For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Chromatin IP | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Rat, Bovine
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human E2F1. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The E2F transcription factors are essential for regulation of the cell cycle (1,2). Physiological E2F is a heterodimer composed of an E2F subunit together with a DP subunit (3, 4). Six members of the E2F family have been identified, and each E2F subunit has a DNA binding and a dimerization domain. E2F-1 to -5 activate transcription. E2F-1 to -3 bind pRb, and E2F-4 and -5 bind p107 or p130, and these interactions are under cell cycle control (5-8). E2F-1 has oncogenic properties in vivo and in vitro. E2F-1 can induce apoptosis through p53-dependent and -independent mechanisms. E2F-1 is stress-responsive, and is regulated by a PI3-kinase-like kinase family such as the ATM/ATR kinases (9-11).
- Helin, K. (1998) Curr. Opin. Genet. Dev. 8, 28-35.
- Dyson, N. (1998) Genes Dev. 12, 2245-2262.
- Helin, K. et al. (1993) Genes Dev. 7, 1850-1861.
- Wu, C. et al. (1995) Mol. Cell. Biol. 15, 2536-2546.
- Takahashi, Y. et al. (2000) Genes Dev. 14, 804-816.
- Wu, L. et al. (2001) Nature 414, 457-462.
- Gaubatz, S. et al. (2000) Mol. Cell 6, 729-735.
- Hurford, R. K. et al. (1997) Genes Dev. 11, 1447-1463.
- Tsai, K. Y. et al. (1998) Mol. Cell 2, 293-304.
- Garcia, I. et al. (2000) Cell Growth Differ. 11, 91-98.
- Lin, W. C. et al. (2001) Genes Dev. 15, 1833-1844.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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