WB
H M R
Endogenous
62
Rabbit IgG
#Q9H9T3
55140
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Hamster, Bovine, Dog, Pig, Horse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly431 of human ELP3 protein.
Background
Elongator is a highly conserved transcription elongation factor complex that was first identified in yeast as part of the hyperphosphorylated RNA polymerase II (RNAPII) holoenzyme (1). The Elongator complex consists of 6 subunits, ELP1-6, and has been shown to have acetyltransferase activity (2). The acetylation targets of Elongator include histone H3, which is linked to the transcription elongation function of the complex, and α-tubulin, which is associated with regulation of migration and maturation of projection neurons (3-6).
ELP3 is the catalytic acetyltransferase subunit of the Elongator complex (2,3). ELP3 contains an iron-sulfur cluster that can bind S-adenosylmethionine, which is necessary for the structural integrity of the Elongator and has been shown to play an important role in mediating global zygotic DNA demethylation of the paternal genome post-fertilization (7-9).
- Otero, G. et al. (1999) Mol Cell 3, 109-18.
- Creppe, C. and Buschbeck, M. (2011) J Biomed Biotechnol 2011, 924898.
- Wittschieben, B.O. et al. (1999) Mol Cell 4, 123-8.
- Hawkes, N.A. et al. (2002) J Biol Chem 277, 3047-52.
- Kim, J.H. et al. (2002) Proc Natl Acad Sci USA 99, 1241-6.
- Creppe, C. et al. (2009) Cell 136, 551-64.
- Paraskevopoulou, C. et al. (2006) Mol Microbiol 59, 795-806.
- Greenwood, C. et al. (2009) J Biol Chem 284, 141-9.
- Okada, Y. et al. (2010) Nature 463, 554-8.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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