IF-F, IF-IC, FC-L
M
Endogenous
Rabbit IgG
#Q9R0H2
59308
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
| Flow Cytometry (Live) | 1:50 - 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of mouse EMCN protein.
Background
Endomucin (EMCN) is a type I transmembrane O-sialoglycoprotein expressed in endothelium in venules and capillaries, but not arteries (1,2). EMCN prevents leukocyte adhesion in non-inflamed tissues, and downregulation of EMCN during inflammation facilitates migration of inflammatory cells into target tissues (3). Notch activation in endothelial cells downregulates EMCN and promotes migration of neutrophils, modulating acute inflammation in hepatic ischemia/reperfusion injury (4). EMCN deficiency in endothelial cells drives tumor lung metastasis by providing a premetastatic niche for cancer cell colonization, and is a potential prognostic biomarker for highly metastatic cancers (5). Combined analysis of EMCN/MUC15 has been suggested as a prognostic signature for gastric cancer (6).
- Kuhn, A. et al. (2002) J Invest Dermatol 119, 1388-93.
- dela Paz, N.G. and D'Amore, P.A. (2009) Cell Tissue Res 335, 5-16.
- Zahr, A. et al. (2016) Nat Commun 7, 10363.
- Zhang, P. et al. (2020) Sci China Life Sci 63, 375-387.
- Zhang, G. et al. (2022) J Transl Med 20, 446.
- Dai, W. et al. (2020) Front Mol Biosci 7, 19.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry) FC-L: Flow Cytometry (Live)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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