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12693
Endodermal Lineage Marker Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Endodermal Lineage Marker Antibody Sampler Kit #12693

Citations (0)
Endodermal Lineage Marker Antibody Sampler Kit: Image 1
Western blot analysis of extract from HepG2 cells using HNF4α (C11F12) Rabbit mAb.
Endodermal Lineage Marker Antibody Sampler Kit: Image 2
Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma using HNF4α (C11F12) Rabbit mAb.
Endodermal Lineage Marker Antibody Sampler Kit: Image 3
Immunohistochemical analysis of paraffin-embedded HepG2 cells in the presence of control peptide (left) or antigen specific peptide (right) using HNF4α (C11F12E8) Rabbit mAb.
Endodermal Lineage Marker Antibody Sampler Kit: Image 4
Confocal immunofluorescent analysis of HepG2 (left) and HeLa cells (right) using HNF4α (C11F12) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
Endodermal Lineage Marker Antibody Sampler Kit: Image 5
Western blot analysis of extracts from A431, MCF-7, C2C12, HUVEC, BAEC, C6 and H-4-II-E cells, using N-cadherin Antibody.
Endodermal Lineage Marker Antibody Sampler Kit: Image 6
Western blot analysis of extracts from Hep G2 cells using AFP (D12C1) Rabbit mAb.
Endodermal Lineage Marker Antibody Sampler Kit: Image 7
Western blot analysis of extracts from E6.5 and E10.5 stage mouse embryos using EOMES Antibody.
Endodermal Lineage Marker Antibody Sampler Kit: Image 8
Western blot analysis of extracts from various cell lines using PDGF Receptor α (D13C6) XP® Rabbit mAb (upper), PDGF Receptor β (28E1) Rabbit mAb #3169 (middle), and β-Actin Antibody #4967 (lower).
Endodermal Lineage Marker Antibody Sampler Kit: Image 9
Immunohistochemical analysis of paraffin-embedded human astrocytoma using PDGF Receptor α (D13C6) XP® Rabbit mAb.
Endodermal Lineage Marker Antibody Sampler Kit: Image 10
Immunohistochemical analysis of paraffin-embedded NCI-H1703 (PDGFRα+, left) and HCC827 (PDGFRα-, right) cell pellets using PDGF Receptor α (D13C6) XP® Rabbit mAb.
Endodermal Lineage Marker Antibody Sampler Kit: Image 11
Immunohistochemical analysis of paraffin-embedded human leiomyosarcoma using PDGF Receptor α (D13C6) XP® Rabbit mAb.
Endodermal Lineage Marker Antibody Sampler Kit: Image 12
Confocal immunofluorescent analysis of NCI-H1703 (left), A172 (center) and HCC827 cells (right) using PDGF Receptor α (D13C6) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Endodermal Lineage Marker Antibody Sampler Kit: Image 13
Flow cytometric analysis of A-204 cells using PDGF Receptor α (D13C6) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Endodermal Lineage Marker Antibody Sampler Kit: Image 14
CUT&RUN was performed with Caco-2 cells and GATA-6 (D61E4) XP® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across FGFR2 gene.
Endodermal Lineage Marker Antibody Sampler Kit: Image 15
Chromatin immunoprecipitations were performed with cross-linked chromatin from Caco-2 cells and GATA-6 (D61E4) XP® Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across FGFR2, a known target gene of GATA-6 (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.
Endodermal Lineage Marker Antibody Sampler Kit: Image 16
Western blot analysis of extracts from Huh7 and 293 cells using GATA-6 (D61E4) XP® Rabbit mAb.
Endodermal Lineage Marker Antibody Sampler Kit: Image 17
CUT&RUN was performed with Caco-2 cells and GATA-6 (D61E4) XP® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figures show binding across chromosome 10 (upper), including FGFR2 gene (lower).
Endodermal Lineage Marker Antibody Sampler Kit: Image 18
Chromatin immunoprecipitations were performed with cross-linked chromatin from Caco-2 cells and GATA-6 (D61E4) XP® Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across chromosome 10 (upper), including FGFR2 (lower), a known target gene of GATA-6 (see additional figure containing ChIP-qPCR data).
Endodermal Lineage Marker Antibody Sampler Kit: Image 19
Confocal immunofluorescent analysis of KM12 (left) and SK-OV-3 cells (right) using GATA-6 (D61E4) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Endodermal Lineage Marker Antibody Sampler Kit: Image 20
CUT&RUN was performed with Caco-2 cells and either GATA-6 (D61E4) XP® Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using human HNMT upstream primer, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Endodermal Lineage Marker Antibody Sampler Kit: Image 21
Chromatin immunoprecipitations were performed with cross-linked chromatin from Caco-2 cells and either GATA-6 (D61E4) XP® Rabbit mAb, or Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human FDPS Promoter Primers #13840, human FGFR2 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Endodermal Lineage Marker Antibody Sampler Kit: Image 22
Flow cytometric analysis of SKOV3 cells (blue) and HUH-7 cells (green) using GATA-6 (D61E4) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor 488 Conjugate) #4412 was used as a secondary antibody.
Endodermal Lineage Marker Antibody Sampler Kit: Image 23
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Endodermal Lineage Marker Antibody Sampler Kit: Image 24
Western blot analysis of extracts from Hep G2 cells using FoxA2/HNF3β (D56D6) XP® Rabbit mAb.
Endodermal Lineage Marker Antibody Sampler Kit: Image 25
Confocal immunofluorescent analysis of Hep G2 (left) and HeLa (right) cells using FoxA2/HNF3β (D56D6) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Endodermal Lineage Marker Antibody Sampler Kit: Image 26
Western blot analysis of extracts from NCCIT cells using Sall4 (D16H12) Rabbit mAb.
Endodermal Lineage Marker Antibody Sampler Kit: Image 27
Confocal immunofluorescent analysis of NTERA-2 (left) and HeLa cells (right) using Sall4 (D16H12) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Inquiry Info.# 12693

Product Description

The Endodermal Lineage Marker Antibody Sampler Kit provides an economical means of evaluating proteins expressed during endoderm development. This kit contains enough antibody to perform four western blot experiments per primary antibody.

Specificity / Sensitivity

Each antibody recognizes endogenous total levels of its specific target protein. Sall4 (D16H12) Rabbit mAb recognizes endogenous levels of total Sall4A and Sall4B proteins.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AFP protein, residues surrounding Gly138 of human FoxA2/HNF3β protein, residues near the amino terminus of human GATA-6 protein, the sequence of human HNF4α protein, a recombinant protein corresponding to the PDGF receptor α extracellular domain, or residues surrounding Ala311 of human Sall4 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human N-cadherin protein or near the amino terminus of human EOMES protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

Two endodermal lineages develop during mammalian embryogenesis, the primitive endoderm of the blastocyst stage embryo and the definitive endoderm at gastrulation. The primitive endoderm gives rise to extra-embryonic lineages encompassing the visceral and the parietal endoderm. The definitive endoderm contributes to the respiratory and gastrointestinal tracts by forming the epithelial lining of the trachea, esophagus, lungs, stomach and intestines, and is a major component of many glands, including thyroid, thymus, pancreas and liver (1). Understanding molecular mechanisms that regulate early endodermal fates is seminal for the advance of stem cell research as they connect the transition from pluripotency to endoderm specification during mammalian development and contribute to the generation of clinically relevant cell types. FoxA2/HNF3β is a transcription factor essential for development of the endoderm and midline structures in mouse embryos (2,3). EOMES acts during gastrulation to promote the specification of the definitive endoderm (4). Markers of hepatic differentiation in the endoderm include expression of α-fetoprotein (AFP) and N-cadherin (5,6). HNF4α is involved in the differentiation of the visceral endoderm. GATA-6 lies upstream of HNF4 in a transcriptional cascade that regulates differentiation of the visceral endoderm and is also required for the establishment of the endodermally derived bronchial epithelium (7). Sall4 is required for the formation of the primitive endoderm from inner cell mass. It has been reported that extra-embryonic stem cell lines cannot be formed in Sall4-deficient blastocysts (8). PDGF receptor α is expressed in primitive endoderm derivatives throughout embryogenesis (9).
  1. Wells, J.M. and Melton, D.A. (1999) Annu Rev Cell Dev Biol 15, 393-410.
  2. Weinstein, D.C. et al. (1994) Cell 78, 575-88.
  3. Ang, S.L. and Rossant, J. (1994) Cell 78, 561-74.
  4. Costello, I. et al. (2011) Nat Cell Biol 13, 1084-91.
  5. Zhao, D. et al. (2009) PLoS One 4, e6468.
  6. Meier, V. et al. (2006) Comp Hepatol 5, 2.
  7. Morrisey, E.E. et al. (1998) Genes Dev 12, 3579-90.
  8. Elling, U. et al. (2006) Proc Natl Acad Sci U S A 103, 16319-24.
  9. Orr-Urtreger, A. et al. (1992) Development 115, 289-303.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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