WB
H
Endogenous
140
Rabbit IgG
#P22413
5167
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu520 of human ENPP1 protein.
Background
Ectonucleotide pyrophosphatase-phosphodiesterase 1 (ENPP1) is a single-pass, type II transmembrane protein primarily involved in ATP hydrolysis at the plasma membrane. Targeting of ENPP1 to the basolateral cell surface relies on the presence of a carboxy-terminal di-leucine-based signal (1). ENPP1 plays important roles in bone mineralization and soft tissue calcification (2-5). Mutations in the corresponding ENPP1 gene cause generalized arterial calcification in infancy (GACI) and idiopathic infantile arterial calcification (IIAC) (6,7). ENPP1 inhibits insulin receptor function and overexpression of this enzyme causes insulin resistance and glucose intolerance in mice (8,9). Genetic variants of ENPP1 have been associated with obesity and type 2 diabetes (10-12).
- Bello, V. et al. (2001) Mol Biol Cell 12, 3004-15.
- Okawa, A. et al. (1998) Nat Genet 19, 271-3.
- Nakamura, I. et al. (1999) Hum Genet 104, 492-7.
- Harmey, D. et al. (2004) Am J Pathol 164, 1199-209.
- Hessle, L. et al. (2002) Proc Natl Acad Sci USA 99, 9445-9.
- Rutsch, F. et al. (2003) Nat Genet 34, 379-81.
- Ruf, N. et al. (2005) Hum Mutat 25, 98.
- Dong, H. et al. (2005) Diabetes 54, 367-72.
- Maddux, B.A. et al. (2006) Am J Physiol Endocrinol Metab 290, E746-9.
- Meyre, D. et al. (2005) Nat Genet 37, 863-7.
- Keene, K.L. et al. (2008) Diabetes 57, 1057-62.
- Bacci, S. et al. (2007) Curr Opin Clin Nutr Metab Care 10, 403-9.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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