Interested in promotions? | Click here >>
9962
Epitope Tag Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Epitope Tag Antibody Sampler Kit #9962

Reviews ()
Citations (0)
Epitope Tag Antibody Sampler Kit: Image 1

Western blot analysis of extracts from COS-7 cells untransfected (-) and transfected cells overexpressing GST-Bad (+), using GST (91G1) Rabbit mAb (left) and Bad Antibody #9292 (right).

Epitope Tag Antibody Sampler Kit: Image 2

Flow cytometric analysis of 293T cells, mock-transfected (blue) or transfected with a construct expressing Myc-Tag (green), using Myc-Tag (71D10) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Epitope Tag Antibody Sampler Kit: Image 3

293T cells were either untransfected (left panel) or transfected with an HA-tagged human CBP construct (right panel), then treated with Forskolin #3828 (30 µM) . Chromatin immunoprecipitations were performed with cross-linked chromatin from cells and HA-Tag (C29F4) Rabbit mAb, CBP (D9B6) Rabbit mAb, or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Epitope Tag Antibody Sampler Kit: Image 4

293T cells were either untransfected (left panel) or transfected with an His-tagged human Stat3 construct (right panel), then treated with Human Interleukin-6 (hIL-6) #8904 (100 ng/ml, 30 min). Chromatin immunoprecipitations were performed with cross-linked chromatin from cells and His-Tag (D3I1O) XP® Rabbit mAb, Stat3α (D1A5) XP® Rabbit mAb #8768, or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Fos Promoter Primers #4663, human IRF-1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Epitope Tag Antibody Sampler Kit: Image 5

Flow cytometric analysis of 293T cells, mock-transfected (blue, negative) or transfected with a construct expressing DYKDDDDK Tag (green, positive), using DYKDDDDK Tag (9A3) Mouse mAb (solid lines) or concentration-matched Mouse (G3A1) mAb IgG1 IsotypeControl #5415 (dashed lines). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4410 was used as a secondary antibody.

Epitope Tag Antibody Sampler Kit: Image 6

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Epitope Tag Antibody Sampler Kit: Image 7

Confocal immunofluorescent analysis of 293 cells stably expressing Myc-tagged ADORA2A (left) versus wild-type 293 cells (right) using Myc-Tag (71D10) Rabbit mAb (green). Blue = DAPI #4083 (fluorescent DNA dye).

Epitope Tag Antibody Sampler Kit: Image 8

Flow cytometric analysis of 293T cells untransfected (blue) or transfected with HA-tagged Akt (green), using HA-Tag (C29F4) Rabbit mAb (solid lines) or concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines).

Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Epitope Tag Antibody Sampler Kit: Image 9

Flow cytometric analysis of 293T cells, untransfected (blue) or transfected with a His-myc-Akt plasmid (green), using His-Tag (D3I1O) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.

Epitope Tag Antibody Sampler Kit: Image 10

Confocal immunofluorescent analysis of HeLa cells transfected with a DYKDDDDK-tagged IRF3 construct using DYKDDDDK Tag (9A3) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Epitope Tag Antibody Sampler Kit: Image 11

Western blot analysis of extracts from untransfected control cells (-) and transfected cells overexpressing Myc-Bcl-2 (+), using Bcl-2 Antibody #2872 (left) and Myc-Tag (71D10) Rabbit mAb (right).

Epitope Tag Antibody Sampler Kit: Image 12

Confocal immunofluorescent analysis of COS cells, transfected with an HA-tagged protein (left) or mock-transfected (right), using HA-Tag (C29F4) Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

Epitope Tag Antibody Sampler Kit: Image 13

Confocal immunofluorescent analysis of 293T cells transfected with a His-Tagged protein using His-Tag (D3I1O) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

Epitope Tag Antibody Sampler Kit: Image 14

Immunohistochemical analysis of paraffin-embedded 293T cell pellets, control (left) or ROS-DYKDDDDK Tag transfected (right), using DYKDDDDK Tag (9A3) Mouse mAb.

Epitope Tag Antibody Sampler Kit: Image 15

Immunohistochemical analysis of paraffin-embedded COS cells, untransfected (right) or HA-Tag transfected (left), using HA-Tag (C29F4) Rabbit mAb.

Epitope Tag Antibody Sampler Kit: Image 16

Immunoprecipitation of His-Tag protein from transfected 293T cells using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or His-Tag (D3I1O) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using His-Tag (D3I1O) XP® Rabbit mAb.

Epitope Tag Antibody Sampler Kit: Image 17

Immunoprecipitation of PI3K-DYKDDDDK tag protein from 293 transfected cell extracts. Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb IgG1 Isotype Control #5415, and lane 3 is DYKDDDDK Tag (9A3) Mouse mAb. Western blot analysis was performed using DYKDDDDK Tag (D6W5B) Rabbit mAb #14793. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.

Epitope Tag Antibody Sampler Kit: Image 18

Western blot analysis of extracts from HeLa cells, untransfected or transfected with either HA-FoxO4 or HA-Akt3, using HA-Tag (C29F4) Rabbit mAb.

Epitope Tag Antibody Sampler Kit: Image 19

Western blot analysis of extracts from 293T cells, untransfected or transfected with 6xHis-Tag fusion protein 1 or 6xHis-Tag fusion protein 2, using His-Tag (D3I1O) XP® Rabbit mAb.

Epitope Tag Antibody Sampler Kit: Image 20

Western blot analysis of extracts from 293T cells, untransfected or transfected with either DYKDDDDK-tagged FoxG1, NPAS4, or Tyrosine Hydroxylase (TH), using DYKDDDDK Tag (9A3) Mouse mAb.

To Purchase # 9962T
Product # Size Price
9962T
1 Kit  (5 x 20 µl) $ 391

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
GST (91G1) Rabbit mAb 2625 20 µl
  • WB
  • IP
All Rabbit IgG
Myc-Tag (71D10) Rabbit mAb 2278 20 µl
  • WB
  • IP
  • IF
  • F
All Rabbit IgG
HA-Tag (C29F4) Rabbit mAb 3724 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
  • ChIP
All Rabbit IgG
His-Tag (D3I1O) XP® Rabbit mAb 12698 20 µl
  • WB
  • IP
  • IF
  • F
  • ChIP
All Rabbit IgG
DYKDDDDK Tag (9A3) Mouse mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) 8146 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
Mouse IgG1
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 

Product Description

The Epitope Tag Antibody Sampler Kit provides an economical means to analyze the expression of a variety of epitope tagged proteins. The kit contains enough primary and secondary antibodies to perform two Western blots per primary antibody.

Specificity / Sensitivity

All antibodies in the Epitope Tag Antibody Sampler Kit detect overexpressed fusion proteins containing the corresponding epitope tags. DYKDDDDK Tag Antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma’s Anti-FLAG® antibodies), and its binding specificity is NOT dependent on the presence of divalent metal cations.

Source / Purification

Rabbit monoclonal antibodies are producted by immunizing rabbits with a GST fusion protein, a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL), residues of the 6xHis epitope tag, or with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA). Mouse monoclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Polyclonal antibodies are produced by immunizing rabbits with a synthetic DYKDDDDK peptide. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

Several different epitope tags are now commonly utilized and readily available. For instance, a variety of plasmids contain DNA that encodes an amino-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography (1). As is the case with other protein tag systems (2), this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest (1). Glutathione S-transferase (GST) is another widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (3-5). The HA tag, derived from an epitope of the influenza hemagglutinin protein, has also been extensively used as a general epitope tag in expression vectors (6), while the Myc epitope tag is routinely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (7). Finally, the DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors and consists of only eight amino acids. This peptide can be expressed and detected with the protein of interested as an amino-terminal or carboxy-terminal fusion (8).

  1. Kroll, D.J. et al. (1993) DNA Cell Biol 12, 441-53.
  2. di Guan, C. et al. (1988) Gene 67, 21-30.
  3. Guan, K.L. and Dixon, J.E. (1991) Anal. Biochem. 192, 262-267.
  4. Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
  5. Yu, J. et al. (1998) Mol. Cell. Biol. 18, 1379-1387.
  6. Field, J. et al. (1988) Mol Cell Biol 8, 2159-65.
  7. Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93.
  8. Brizzard, B.L. et al. (1994) Biotechniques 16, 730-5.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.