WB, IHC-P, IF-IC
H M Mk
Endogenous
123
Rabbit IgG
#O14980
7514
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human exportin-1/CRM1 protein.
Background
Exportins are a family of seven proteins that are responsible for intracellular transport. Exportin-1, also known as chromosome region maintenance 1 (CRM1), is a protein essential for nuclear export of hundreds of proteins, mRNAs, and rRNAs (1-3). Exportin-1 binds to substrates with nuclear export signals (NESs) rich in leucine and other hydrophobic amino acids (4). These hydrophobic sequences form an alpha-helix-loop that can bind to the exportin-1 hydrophobic groove (5). Studies have shown that these NESs can be modified either by protein modifications or by mutation to regulate exportin-1 binding (6-7). Targets of exportin-1 include many tumor suppressors, such as Rb, p53, FoxO1, BAF47, as well as oncoproteins, such as p21 and p27 (1). In addition, Myc can upregulate exportin-1 during biogenesis, where it can export newly formed 40S and 60S subunits from the nucleoli (8-9).
Inhibition of nuclear export has been pursued for therapeutic application since the finding that leptomycin B could suppress HIV replication by suppressing the ability of exportin-1 to export the HIV-1 protein Rev (2, 10). Overexpression of exportin-1 has been associated with poor prognosis in various cancer types (11-13). Genomic approaches and development of inhibitors have identified exportin-1 as a druggable target (14-16). The use of various inhibitors of exportin-1 is also being explored in various antiviral therapies (17-18).
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- Fukuda, M. et al. (1997) Nature 390, 308-11.
- Kutay, U. and Güttinger, S. (2005) Trends Cell Biol 15, 121-4.
- Dong, X. et al. (2009) Nat Struct Mol Biol 16, 558-60.
- Vogt, P.K. et al. (2005) Cell Cycle 4, 908-13.
- Craig, E. et al. (2002) EMBO J 21, 31-42.
- Thomas, F. and Kutay, U. (2003) J Cell Sci 116, 2409-19.
- Golomb, L. et al. (2012) Mol Cell 45, 222-32.
- Wolff, B. et al. (1997) Chem Biol 4, 139-47.
- Noske, A. et al. (2008) Cancer 112, 1733-43.
- Shen, A. et al. (2009) Neurosurgery 65, 153-9; discussion 159-60.
- Yao, Y. et al. (2009) Oncol Rep 21, 229-35.
- Schmidt, J. et al. (2013) Leukemia 27, 2357-65.
- Boyle, S.M. et al. (1999) J Virol 73, 6872-81.
- Walker, C.J. et al. (2013) Blood 122, 3034-44.
- Yadav, V. et al. (2016) Virusdisease 27, 357-368.
- Chutiwitoonchai, N. et al. (2017) Virology 507, 32-39.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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