| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| COL1A1 (E8F4L) XP® Rabbit mAb | 72026 | 20 µl | 220 kDa | Rabbit IgG |
| Tenascin C (E5J3B) Rabbit mAb | 33352 | 20 µl | 200, 240 kDa | Rabbit IgG |
| Thrombospondin-1 (D7E5F) Rabbit mAb | 37879 | 20 µl | 170 kDa | Rabbit IgG |
| Fibronectin/FN1 (E5H6X) Rabbit mAb | 26836 | 20 µl | 300 kDa | Rabbit IgG |
| COL4A1 Antibody | 50273 | 20 µl | 200 kDa | Rabbit |
| SPARC (D10F10) Rabbit mAb | 8725 | 20 µl | 42 kDa | Rabbit IgG |
| Periostin (E5F2S) Rabbit mAb | 20302 | 20 µl | 90 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat | |
| COL3A1 (E8D7R) XP® Rabbit mAb | 63034 | 20 µl | 200 kDa | Rabbit IgG |
| CYR61 (D4H5D) XP® Rabbit mAb | 14479 | 20 µl | 41 kDa | Rabbit IgG |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Extracellular Matrix Dynamics Antibody Sampler Kit provides an economical means of detecting selected proteins associated with dynamic remodeling of the extracellular matrix. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Storage
Background
The extracellular matrix (ECM) is a three-dimensional macromolecular network composed of collagens, proteoglycans, glycosaminoglycans, elastin, fibronectin, laminins, along with many other proteins and glycoproteins. This network of macromolecules provides a dynamic microenvironment that supports cell and tissue function, and undergoes continuous remodeling during both normal development and disease (1). Remodeling of the ECM can alter the relative balance of macromolecules within distinct ECM subcompartments, with important functional consequences; for example, changes to the relative amounts of COL1A1 and COL3A1 in the interstitial ECM, or COL4A1 and laminins in the basement membrane, can influence cell-matrix interactions, and/or disrupt cellular signaling events (2). Fibronectin functions as a physical and functional bridge between many different ECM components, including collagens, growth factors, and cell surface integrins, and thus plays a critical role in facilitating ECM remodeling. Matricellular proteins (MCPs) are another important group of ECM proteins. MCPs can be categorized into 6 distinct subgroups: centralized coordination network (CCN), thrombospondin (THBS), secreted protein acidic and rich in cysteine (SPARC), tenascin (TN), small integrin-binding ligand N-linked glycoprotein (SIBLING), and γ-carboxyglutamate (Gla)-containing proteins. CCN1 (CYR61), SPARC, tenascin C, periostin, and thrombospondin-1 are among the most well-studied of this group. All are non-structural ECM proteins that interact with structural ECM proteins, in part to regulate the rigidity of the ECM. They also play important roles in matrix-cell communication by engaging with cell surface receptors and integrins to elicit intracellular responses. The dysregulation of MCP expression has been associated with the development of numerous disease states, including cancer and fibrosis (4,5).
- Bonnans, C. et al. (2014) Nat Rev Mol Cell Biol 15, 786-801.
- Theocharis, A.D. et al. (2019) FEBS J 286, 2830-2869.
- Zollinger, A.J. and Smith, M.L. (2017) Matrix Biol 60-61, 27-37.
- Gerarduzzi, C. et al. (2020) Cancer Res 80, 2705-2717.
- Feng, D. and Gerarduzzi, C. (2020) Int J Mol Sci 21, 4776. doi: 10.3390/ijms21134776.
Background References
Trademarks and Patents
Limited Uses
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