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Support:

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3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Quantity Mol. Wt Isotype/Source
COL1A1 (E8F4L) Rabbit Monoclonal Antibody7202620 µl220 kDaRabbit IgG
Tenascin C (E5J3B) Rabbit Monoclonal Antibody3335220 µl200, 240 kDaRabbit IgG
Thrombospondin-1 (D7E5F) Rabbit Monoclonal Antibody3787920 µl170 kDaRabbit IgG
Fibronectin/FN1 (E5H6X) Rabbit Monoclonal Antibody2683620 µl300 kDaRabbit IgG
COL4A1 Antibody5027320 µl200 kDaRabbit
SPARC (D10F10) Rabbit Monoclonal Antibody872520 µl42 kDaRabbit IgG
Periostin (E5F2S) Rabbit Monoclonal Antibody2030220 µl90 kDaRabbit IgG
Anti-rabbit IgG, HRP-linked Antibody7074100 µlGoat
COL3A1 (E8D7R) Rabbit Monoclonal Antibody6303420 µl200 kDaRabbit IgG
CYR61 (D4H5D) Rabbit Monoclonal Antibody1447920 µl41 kDaRabbit IgG

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Extracellular Matrix Dynamics Antibody Sampler Kit provides an economical means of detecting selected proteins associated with dynamic remodeling of the extracellular matrix. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.

Background

The extracellular matrix (ECM) is a three-dimensional macromolecular network composed of collagens, proteoglycans, glycosaminoglycans, elastin, fibronectin, laminins, along with many other proteins and glycoproteins. This network of macromolecules provides a dynamic microenvironment that supports cell and tissue function, and undergoes continuous remodeling during both normal development and disease (1). Remodeling of the ECM can alter the relative balance of macromolecules within distinct ECM subcompartments, with important functional consequences; for example, changes to the relative amounts of COL1A1 and COL3A1 in the interstitial ECM, or COL4A1 and laminins in the basement membrane, can influence cell-matrix interactions, and/or disrupt cellular signaling events (2). Fibronectin functions as a physical and functional bridge between many different ECM components, including collagens, growth factors, and cell surface integrins, and thus plays a critical role in facilitating ECM remodeling. Matricellular proteins (MCPs) are another important group of ECM proteins. MCPs can be categorized into 6 distinct subgroups: centralized coordination network (CCN), thrombospondin (THBS), secreted protein acidic and rich in cysteine (SPARC), tenascin (TN), small integrin-binding ligand N-linked glycoprotein (SIBLING), and γ-carboxyglutamate (Gla)-containing proteins. CCN1 (CYR61), SPARC, tenascin C, periostin, and thrombospondin-1 are among the most well-studied of this group. All are non-structural ECM proteins that interact with structural ECM proteins, in part to regulate the rigidity of the ECM. They also play important roles in matrix-cell communication by engaging with cell surface receptors and integrins to elicit intracellular responses. The dysregulation of MCP expression has been associated with the development of numerous disease states, including cancer and fibrosis (4,5).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using CYR61 (D4H5D) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, MCF7 cells are negative for CYR61 expression.
undefined Image 1: Extracellular Matrix Dynamics Antibody Sampler Kit
Western blot analysis of extracts from Hs 578T and AN3-CA cells using Periostin (E5F2S) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of periostin protein in AN3-CA cells is consistent with the predicted expression pattern.
undefined Image 2: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunoprecipitation of periostin protein from Hs 578T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Periostin (E5F2S) Rabbit mAb. Western blot analysis was performed using Periostin (E5F2S) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody.
undefined Image 3: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using Periostin (E5F2S) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of periostin protein in SW620 cells is consistent with the predicted expression pattern.
undefined Image 4: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Periostin (E5F2S) Rabbit mAb.
undefined Image 5: Extracellular Matrix Dynamics Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using Fibronectin/FN1 (E5H6X) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). The absence of Fibronectin/FN1 expression in PANC-1 cell extracts is consistent with molecular and proteomic expression profiling data, confirming specificity of the antibody.
undefined Image 6: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from RH-30 and IGROV-1 cells using Tenascin C (E5J3B) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). The differential in Tenascin C expression between RH-30 and IGROV-1 cells is consistent with their reported molecular expression profiles (CCLE, portals.broadinstitute.org), confirming specificity of the antibody for Tenascin C.
undefined Image 7: Extracellular Matrix Dynamics Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using Thrombospondin-1 (D7E5F) Rabbit mAb.
undefined Image 8: Extracellular Matrix Dynamics Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using COL4A1 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). The absence of COL4A1 expression in HCT 116 cell extracts is consistent with molecular and proteomic expression profiling data, confirming specificity of the antibody.
undefined Image 9: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Western blot analysis of extracts from RD and ACHN cells using COL3A1 (E8D7R) Rabbit Monoclonal Antibody (upper) and β-Actin (D6A8) Rabbit Monoclonal Antibody #8457 (lower). Differential expression of COL3A1 in RD and ACHN cell lines is consistent with molecular and proteomic expression profiling data, confirming the specificity of the antibody for COL3A1.
undefined Image 10: Extracellular Matrix Dynamics Antibody Sampler Kit
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
undefined Image 11: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using COL1A1 (E8F4L) XP® Rabbit mAb performed on the Leica® BOND™ Rx. 
undefined Image 12: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using COL1A1 (E8F4L) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
undefined Image 13: Extracellular Matrix Dynamics Antibody Sampler Kit
Western blot analysis of culture medium, from SPARC-secreting PYS-2 cells, using SPARC (D10F10) Rabbit mAb.
undefined Image 14: Extracellular Matrix Dynamics Antibody Sampler Kit
Confocal immunofluorescent analysis of Saos-2 cells, untreated (left) or treated with Brefeldin A #9972 (10 μg/ml, overnight; center), and untreated MCF7 cells (right), using CYR61 (D4H5D) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4804 (fluorescent DNA dye).
undefined Image 15: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using Periostin (E5F2S) Rabbit mAb.
undefined Image 16: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunoprecipitation of Fibronectin/FN1 protein from Hep G2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Fibronectin/FN1 (E5H6X) Rabbit mAb. Western blot analysis was performed using Fibronectin/FN1 (E5H6X) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
undefined Image 17: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunoprecipitation of Tenascin C protein from U-87 MG cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Tenascin C (E5J3B) Rabbit mAb. Western blot analysis was performed using Tenascin C (E5J3B) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.
undefined Image 18: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunoprecipitation of thrombospondin-1 protein from ACHN cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Thrombospondin-1 (D7E5F) Rabbit mAb. Western blot analysis was performed using Thrombospondin-1 (D7E5F) Rabbit mAb.
undefined Image 19: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using COL1A1 (E8F4L) XP® Rabbit mAb performed on the Leica® BOND™ Rx. 
undefined Image 20: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SPARC (D10F10) Rabbit mAb.
undefined Image 21: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Flow cytometric analysis of 293T cells (blue) and PANC-1 cells (green) using CYR61 (D4H5D) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
undefined Image 22: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using Periostin (E5F2S) Rabbit mAb.
undefined Image 23: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human neuroendocrine carcinoma of the lung using Fibronectin/FN1 (E5H6X) Rabbit mAb performed on the Leica® BOND™ Rx.
undefined Image 24: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of RH-30 cells (left, high-expressing) or HCT 116 cells (right, low-expressing) using Tenascin C (E5J3B) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 25: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using COL1A1 (E8F4L) XP® Rabbit mAb.
undefined Image 26: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using SPARC (D10F10) Rabbit mAb.
undefined Image 27: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using Periostin (E5F2S) Rabbit mAb.
undefined Image 28: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded Hep G2 cell pellet (left, positive) or PANC-1 cell pellet (right, negative) using Fibronectin/FN1 (E5H6X) Rabbit mAb.
undefined Image 29: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human kidney using COL1A1 (E8F4L) XP® Rabbit mAb.
undefined Image 30: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Periostin (E5F2S) Rabbit mAb.
undefined Image 31: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb.
undefined Image 32: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human colon carcinoma (left), renal cell carcinoma (middle) or prostate carcinoma (right) using COL1A1 (E8F4L) XP® Rabbit mAb (top) or COL1A1 (E3E1X) Mouse mAb #66948 (bottom). These two antibodies detect independent, unique epitopes on human COL1A1. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
undefined Image 33: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded 293T cell pellet, untransfected (left) or periostin-transfected (right), using Periostin (E5F2S) Rabbit mAb. 
undefined Image 34: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb.
undefined Image 35: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using COL1A1 (E8F4L) XP® Rabbit mAb.
undefined Image 36: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Periostin (E5F2S) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). 
undefined Image 37: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human adenoid cystic carcinoma of the trachea using Fibronectin/FN1 (E5H6X) Rabbit mAb (left) or Fibronectin Rabbit mAb (right). These two antibodies detect independent, unique epitopes on human Fibronectin/FN1 protein. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
undefined Image 38: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded GL261 syngeneic tumor using COL1A1 (E8F4L) XP® Rabbit mAb.
undefined Image 39: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded Hs 578T cell pellet (left, positive) or AN3-CA cell pellet (right, negative) using Periostin (E5F2S) Rabbit mAb. 
undefined Image 40: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
undefined Image 41: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded mouse liver using COL1A1 (E8F4L) XP® Rabbit mAb.
undefined Image 42: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb.
undefined Image 43: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded mouse lung using COL1A1 (E8F4L) XP® Rabbit mAb.
undefined Image 44: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using Fibronectin/FN1 (E5H6X) Rabbit mAb.
undefined Image 45: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded rhesus monkey heart using COL1A1 (E8F4L) XP® Rabbit mAb.
undefined Image 46: Extracellular Matrix Dynamics Antibody Sampler Kit
Confocal immunofluorescent analysis of U-118 MG cells (left, positive) and PANC-1 cells (right, negative) using Fibronectin/FN1 (E5H6X) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 650 Phalloidin #12956 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 47: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded rat colon using COL1A1 (E8F4L) XP® Rabbit mAb.
undefined Image 48: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using COL1A1 (E8F4L) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
undefined Image 49: Extracellular Matrix Dynamics Antibody Sampler Kit
Confocal immunofluorescent analysis of fixed frozen mouse spleen labeled with COL1A1 (E8F4L) Rabbit Monoclonal Antibody (green). Free secondary binding sites were then blocked with Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control #3900 prior to co-labeling with alpha-Smooth Muscle Actin (D4K9N) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate) #76113 (red) and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 50: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human colon carcinoma (left), renal cell carcinoma (middle) or prostate carcinoma (right) using COL1A1 (E8F4L) XP® Rabbit mAb (top) or COL1A1 (E3E1X) Mouse mAb #66948 (bottom). These two antibodies detect independent, unique epitopes on human COL1A1. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
undefined Image 51: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded U-118 MG cell pellet (left, positive) or HT-29 cell pellet (right, negative) using COL1A1 (E8F4L) XP® Rabbit mAb.
undefined Image 52: Extracellular Matrix Dynamics Antibody Sampler Kit
Confocal immunofluorescent analysis of fixed frozen mouse colon labeled with COL1A1 (E8F4L) Rabbit Monoclonal Antibody (green). Free secondary binding sites were then blocked with Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control #3900 prior to co-labeling with beta-Catenin (D10A8) Rabbit Monoclonal Antibody (Alexa Fluor® 555 Conjugate) #83539 (yellow), alpha-Smooth Muscle Actin (D4K9N) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate) #76113 (red), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 53: Extracellular Matrix Dynamics Antibody Sampler Kit
Confocal immunofluorescent analysis of fixed frozen mouse liver labeled with COL1A1 (E8F4L) Rabbit Monoclonal Antibody (green). Free secondary binding sites were then blocked with Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control #3900 prior to co-labeling with alpha-Smooth Muscle Actin (D4K9N) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate) #76113 (red) and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 54: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Confocal immunofluorescent analysis of U-118 MG cells (left, positive) and HT-29 cells (right, negative) using COL1A1 (E8F4L) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
undefined Image 55: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using COL3A1 (E8D7R) Rabbit Monoclonal Antibody (left) compared to concentration-matched Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control #3900 (right).
undefined Image 56: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded RD cell pellet (left, positive) or ACHN cell pellet (right, negative) using COL3A1 (E8D7R) Rabbit Monoclonal Antibody.
undefined Image 57: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using COL3A1 (E8D7R) Rabbit Monoclonal Antibody.
undefined Image 58: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human kidney using COL3A1 (E8D7R) Rabbit Monoclonal Antibody.
undefined Image 59: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using COL3A1 (E8D7R) Rabbit Monoclonal Antibody.
undefined Image 60: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded human large cell neuroendocrine lung carcinoma using COL3A1 (E8D7R) Rabbit Monoclonal Antibody.
undefined Image 61: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human lung sarcoma using COL3A1 (E8D7R) Rabbit Monoclonal Antibody.
undefined Image 62: Extracellular Matrix Dynamics Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using COL3A1 (E8D7R) Rabbit Monoclonal Antibody.
undefined Image 63: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor using COL3A1 (E8D7R) Rabbit Monoclonal Antibody.
undefined Image 64: Extracellular Matrix Dynamics Antibody Sampler Kit
Confocal immunofluorescent analysis of RD cells (left, positive) and ACHN cells (right, negative) using COL3A1 (E8D7R) Rabbit Monoclonal Antibody (green), DyLight 554 Phalloidin #13054 (red), and DAPI #4083 (blue).
undefined Image 65: Extracellular Matrix Dynamics Antibody Sampler Kit
Simple WesternTM analysis of lysates (0.1 mg/mL) from U-118MG cells COL1A1 (E8F4L) XP® Rabbit mAb #72026.  The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody.  The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody.  This experiment was performed under reducing conditions on the JessTM Simple Western instrument from ProteinSimple, a BioTechne brand, using the 66-440 kDa separation module.
undefined Image 66: Extracellular Matrix Dynamics Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.