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63387
Extracellular Matrix Dynamics Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Extracellular Matrix Dynamics Antibody Sampler Kit #63387

Citations (0)
Western blot analysis of extracts from various cell lines using CYR61 (D4H5D) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, MCF7 cells are negative for CYR61 expression.
Western blot analysis of extracts from Hs 578T and AN3-CA cells using Periostin (E5F2S) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of periostin protein in AN3-CA cells is consistent with the predicted expression pattern.
Immunoprecipitation of periostin protein from Hs 578T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Periostin (E5F2S) Rabbit mAb. Western blot analysis was performed using Periostin (E5F2S) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody.
Western blot analysis of extracts from various cell lines using Periostin (E5F2S) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of periostin protein in SW620 cells is consistent with the predicted expression pattern.
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Periostin (E5F2S) Rabbit mAb.
Western blot analysis of extracts from various cell lines using Fibronectin/FN1 (E5H6X) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). The absence of Fibronectin/FN1 expression in PANC-1 cell extracts is consistent with molecular and proteomic expression profiling data, confirming specificity of the antibody.
Western blot analysis of extracts from RH-30 and IGROV-1 cells using Tenascin C (E5J3B) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). The differential in Tenascin C expression between RH-30 and IGROV-1 cells is consistent with their reported molecular expression profiles (CCLE, portals.broadinstitute.org), confirming specificity of the antibody for Tenascin C.
Western blot analysis of extracts from various cell lines using Thrombospondin-1 (D7E5F) Rabbit mAb.
Western blot analysis of extracts from various cell lines using COL4A1 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). The absence of COL4A1 expression in HCT 116 cell extracts is consistent with molecular and proteomic expression profiling data, confirming specificity of the antibody.
Western blot analysis of extracts from RD and ACHN cells using COL3A1 (E8D7R) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Differential expression of COL3A1 in RD and ACHN cell lines is consistent with molecular and proteomic expression profiling data, confirming specificity of the antibody for COL3A1.
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using COL3A1 (E8D7R) XP® Rabbit mAb.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from various cell lines using COL1A1 (E8F4L) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western blot analysis of culture medium, from SPARC-secreting PYS-2 cells, using SPARC (D10F10) Rabbit mAb.
Confocal immunofluorescent analysis of Saos-2 cells, untreated (left) or treated with Brefeldin A #9972 (10 μg/ml, overnight; center), and untreated MCF7 cells (right), using CYR61 (D4H5D) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4804 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using Periostin (E5F2S) Rabbit mAb.
Immunoprecipitation of Fibronectin/FN1 protein from Hep G2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Fibronectin/FN1 (E5H6X) Rabbit mAb. Western blot analysis was performed using Fibronectin/FN1 (E5H6X) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Immunoprecipitation of Tenascin C protein from U-87 MG cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Tenascin C (E5J3B) Rabbit mAb. Western blot analysis was performed using Tenascin C (E5J3B) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.
Immunoprecipitation of thrombospondin-1 protein from ACHN cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Thrombospondin-1 (D7E5F) Rabbit mAb. Western blot analysis was performed using Thrombospondin-1 (D7E5F) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor using COL3A1 (E8D7R) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using COL1A1 (E8F4L) XP® Rabbit mAb performed on the Leica® BOND Rx. 
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SPARC (D10F10) Rabbit mAb.
Flow cytometric analysis of 293T cells (blue) and PANC-1 cells (green) using CYR61 (D4H5D) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using Periostin (E5F2S) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human neuroendocrine carcinoma of the lung using Fibronectin/FN1 (E5H6X) Rabbit mAb performed on the Leica® BOND Rx.
Confocal immunofluorescent analysis of RH-30 cells (left, high-expressing) or HCT 116 cells (right, low-expressing) using Tenascin C (E5J3B) Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunohistochemical analysis of paraffin-embedded human lung sarcoma using COL3A1 (E8D7R) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using COL1A1 (E8F4L) XP® Rabbit mAb performed on the Leica® BOND Rx. 
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using SPARC (D10F10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using Periostin (E5F2S) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded Hep G2 cell pellet (left, positive) or PANC-1 cell pellet (right, negative) using Fibronectin/FN1 (E5H6X) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human large cell neuroendocrine lung carcinoma using COL3A1 (E8D7R) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using COL1A1 (E8F4L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Periostin (E5F2S) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using COL3A1 (E8D7R) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal human kidney using COL1A1 (E8F4L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded 293T cell pellet, untransfected (left) or periostin-transfected (right), using Periostin (E5F2S) Rabbit mAb. 
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using COL3A1 (E8D7R) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using COL1A1 (E8F4L) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Periostin (E5F2S) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). 
Immunohistochemical analysis of paraffin-embedded human adenoid cystic carcinoma of the trachea using Fibronectin/FN1 (E5H6X) Rabbit mAb (left) or Fibronectin Rabbit mAb (right). These two antibodies detect independent, unique epitopes on human Fibronectin/FN1 protein. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Immunohistochemical analysis of paraffin-embedded normal human kidney using COL3A1 (E8D7R) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse liver using COL1A1 (E8F4L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded Hs 578T cell pellet (left, positive) or AN3-CA cell pellet (right, negative) using Periostin (E5F2S) Rabbit mAb. 
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using COL3A1 (E8D7R) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemical analysis of paraffin-embedded mouse lung using COL1A1 (E8F4L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded RD cell pellet (left, positive) or ACHN cell pellet (right, negative) using COL3A1 (E8D7R) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using COL1A1 (E8F4L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using Fibronectin/FN1 (E5H6X) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded GL261 syngeneic tumor using COL1A1 (E8F4L) XP® Rabbit mAb.
Confocal immunofluorescent analysis of U-118 MG cells (left, positive) and PANC-1 cells (right, negative) using Fibronectin/FN1 (E5H6X) Rabbit mAb (green). Actin filaments were labeled with DyLight 650 Phalloidin #12956 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunohistochemical analysis of paraffin-embedded U-118 MG cell pellet (left, positive) or HT-29 cell pellet (right, negative) using COL1A1 (E8F4L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma (left), renal cell carcinoma (middle) or prostate carcinoma (right) using COL1A1 (E8F4L) XP® Rabbit mAb (top) or COL1A1 (E3E1X) Mouse mAb #66948 (bottom). These two antibodies detect independent, unique epitopes on human COL1A1. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Confocal immunofluorescent analysis of mouse spleen using COL1A1 (E8F4L) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #70034 (cyan pseudocolor) and α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #36110 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Confocal immunofluorescent analysis of mouse colon using COL1A1 (E8F4L) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #70034 (cyan pseudocolor) and α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #36110 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Confocal immunofluorescent analysis of mouse liver using COL1A1 (E8F4L) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #70034 (cyan pseudocolor) and α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #36110 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Confocal immunofluorescent analysis of U-118 MG cells (left, positive) and HT-29 cells (right, negative) using COL1A1 (E8F4L) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
To Purchase # 63387
Cat. # Size Qty. Price
63387T
1 Kit  (9 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
COL1A1 (E8F4L) XP® Rabbit mAb 72026 20 µl
  • WB
  • IHC
  • IF
H M 220 Rabbit IgG
Tenascin C (E5J3B) Rabbit mAb 33352 20 µl
  • WB
  • IP
  • IF
H 200, 240 Rabbit IgG
Thrombospondin-1 (D7E5F) Rabbit mAb 37879 20 µl
  • WB
  • IP
H M R 170 Rabbit IgG
Fibronectin/FN1 (E5H6X) Rabbit mAb 26836 20 µl
  • WB
  • IP
  • IHC
  • IF
H 300 Rabbit IgG
COL4A1 Antibody 50273 20 µl
  • WB
H 200 Rabbit 
SPARC (D10F10) Rabbit mAb 8725 20 µl
  • WB
  • IHC
H M 42 Rabbit IgG
Periostin (E5F2S) Rabbit mAb 20302 20 µl
  • WB
  • IP
  • IHC
H 90 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Rab Goat 
COL3A1 (E8D7R) XP® Rabbit mAb 63034 20 µl
  • WB
  • IHC
H 200 Rabbit IgG
CYR61 (D4H5D) XP® Rabbit mAb 14479 20 µl
  • WB
  • IF
  • F
H 41 Rabbit IgG

Product Description

The Extracellular Matrix Dynamics Antibody Sampler Kit provides an economical means of detecting selected proteins associated with dynamic remodeling of the extracellular matrix. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Extracellular Matrix Dynamics Antibody Sampler Kit detects endogenous levels of its target protein. Periostin (E5F2S) Rabbit mAb recognizes endogenous levels of total human periostin protein. The antibody also weakly detects a 40 kDa protein of unknown identity.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with recombinant proteins specific to the carboxy terminus of human Tenascin C protein and human Fibronectin/FN1 protein; with synthetic peptides corresponding to residues surrounding Phe1197 of human COL1A1 protein, Ser395 of human periostin protein, and Pro171 of human CYR61 protein, the amino terminus of human thrombospondin-1 protein and human SPARC protein, and the carboxy terminus of human COL3A1 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp536 of human COL4A1 protein. Antibodies are purified by peptide affinity chromatography.

Background

The extracellular matrix (ECM) is a three-dimensional macromolecular network composed of collagens, proteoglycans, glycosaminoglycans, elastin, fibronectin, laminins, along with many other proteins and glycoproteins. This network of macromolecules provides a dynamic microenvironment that supports cell and tissue function, and undergoes continuous remodeling during both normal development and disease (1). Remodeling of the ECM can alter the relative balance of macromolecules within distinct ECM subcompartments, with important functional consequences; for example, changes to the relative amounts of COL1A1 and COL3A1 in the interstitial ECM, or COL4A1 and laminins in the basement membrane, can influence cell-matrix interactions, and/or disrupt cellular signaling events (2). Fibronectin functions as a physical and functional bridge between many different ECM components, including collagens, growth factors, and cell surface integrins, and thus plays a critical role in facilitating ECM remodeling. Matricellular proteins (MCPs) are another important group of ECM proteins. MCPs can be categorized into 6 distinct subgroups: centralized coordination network (CCN), thrombospondin (THBS), secreted protein acidic and rich in cysteine (SPARC), tenascin (TN), small integrin-binding ligand N-linked glycoprotein (SIBLING), and γ-carboxyglutamate (Gla)-containing proteins. CCN1 (CYR61), SPARC, tenascin C, periostin, and thrombospondin-1 are among the most well-studied of this group. All are non-structural ECM proteins that interact with structural ECM proteins, in part to regulate the rigidity of the ECM. They also play important roles in matrix-cell communication by engaging with cell surface receptors and integrins to elicit intracellular responses. The dysregulation of MCP expression has been associated with the development of numerous disease states, including cancer and fibrosis (4,5).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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