WB, W-S, IP
H M R
Endogenous
45
Rabbit IgG
#Q96E09
116224
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Simple Western™ | 1:10 - 1:50 |
Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala132 of human FAM122A protein.
Background
FAM122A is an inhibitor of serine/threonine-protein phosphatase 2A (PP2A) activity and stability (1). FAM122A promotes the growth of hepatocellular carcinoma (HCC) and acute myeloid leukemia (AML) cells, and increased expression of FAM122A has been reported in HCC and AML cancers (2,3). Chk1 phosphorylates FAM122A at Ser37, inactivating FAM122A, and therefore activating PP2A. PP2A promotes G2/M checkpoint activation through dephosphorylation of the CDK1 inhibitory kinase WEE1. CRISPR knockout of FAM122A confers resistance to inhibitors of Chk1 and ATR in lung cancer cell lines. Expression of FAM122A may serve as a biomarker for sensitivity to Chk1 inhibitors, which are under investigation for the treatment of non-small cell lung cancer (NSCLC) and other cancers (4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting W-S: Simple Western™ IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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