| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| AceCS1 (D19C6) Rabbit mAb | 3658 | 20 µl | 78 kDa | Rabbit IgG |
| Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb | 11818 | 20 µl | 280 kDa | Rabbit IgG |
| Acetyl-CoA Carboxylase (C83B10) Rabbit mAb | 3676 | 20 µl | 280 kDa | Rabbit IgG |
| ATP-Citrate Lyase Antibody | 4332 | 20 µl | 125 kDa | Rabbit |
| Phospho-ATP-Citrate Lyase (Ser455) Antibody | 4331 | 20 µl | 125 kDa | Rabbit |
| Fatty Acid Synthase (C20G5) Rabbit mAb | 3180 | 20 µl | 273 kDa | Rabbit IgG |
| Lipin 1 (D2W9G) Rabbit mAb | 14906 | 20 µl | 130 kDa | Rabbit IgG |
| ACSL1 (D2H5) Rabbit mAb | 9189 | 20 µl | 78 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Fatty Acid and Lipid Metabolism Antibody Sampler Kit provides an economical means to evaluate key proteins involved in fatty acid and lipid metabolism. This kit includes enough primary antibody to perform two western miniblot experiments with each primary antibody.
Storage
Background
The processes of fatty acid and lipid metabolism are vital for cellular nutrient and energy maintenance. Cytoplasmic acetyl-CoA synthetase (AceCS1) catalyzes the conversion of acetate and CoA to acetyl-CoA. Acetyl-CoA synthesized by AceCS1 is used for fatty acid and lipid biosynthesis (1,2). Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). Mammalian long-chain acyl-CoA synthetase (ACSL) catalyzes the ligation of the fatty acid to CoA to form fatty acyl-CoA in a two-step reaction (4). ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol, and acetylcholine, as well as for glucogenesis (5). Phosphorylation of ACL at Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme (6). Fatty acid synthase (FASN) catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA (7). Lipin 1 plays a role in lipid metabolism in various tissues and cell types including liver, muscle, adipose tissues, and neuronal cell lines (8-10). It has dual functions at the molecular level: Lipin 1 serves as a transcriptional coactivator in the liver and a phosphatidate phosphatase in triglyceride and phospholipid biosynthesis pathways (11).
- Ikeda, Y. et al. (2001) J Biol Chem 276, 34259-69.
- Luong, A. et al. (2000) J Biol Chem 275, 26458-66.
- Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
- Mashek, D.G. et al. (2004) J Lipid Res 45, 1958-61.
- Towle, H.C. et al. (1997) Annu Rev Nutr 17, 405-33.
- Potapova, I.A. et al. (2000) Biochemistry 39, 1169-79.
- Katsurada, A. et al. (1990) Eur J Biochem 190, 427-33.
- Finck, B.N. et al. (2006) Cell Metab 4, 199-210.
- Phan, J. and Reue, K. (2005) Cell Metab 1, 73-83.
- Verheijen, M.H. et al. (2003) Genes Dev 17, 2450-64.
- Reue, K. and Zhang, P. (2008) FEBS Lett 582, 90-6.
Background References
Trademarks and Patents
Limited Uses
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