WB, IHC-Bond, IHC-P
H
Endogenous
50
Rabbit IgG
#P02679
2266
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:200 - 1:800 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Storage
For a carrier free (BSA and azide free) version of this product see product #38915.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser190 of human fibrinogen gamma chain protein.
Background
Fibrinogen is a large, multimeric plasma glycoprotein that is constitutively synthesized by liver hepatocytes. As a key component of blood clots, fibrinogen plays a central role in hemostasis and thrombosis. In total, six polypeptide chains (2Aα, 2Bβ, and 2γ) are produced intracellularly in a coordinated manner, which are then covalently linked together by a network of disulfide bonds to form a fibrinogen heterohexamer (1). Research studies have shown that expression of the three fibrinogen genes is upregulated as part of the acute phase inflammatory response (2,3).
Upon engagement of the clotting cascade, thrombin-mediated cleavage of the fibrinogen Aα and Bβ chains creates fibrin monomers and a platform for the polymerization of fibrin monomers into protofibrils and an insoluble web of stable fibrin fibers (4,5). Whereas thrombin is the enzyme that drives fibrin polymerization and clot formation, plasmin is the enzymatic counterpart that facilitates fibrinolysis and clot breakdown (6).
In addition to its position as a central node in the normal coagulation cascade, dysregulated fibrin deposition has been observed in pathological conditions such as cancer and viral infection (7,8).
The fibrinogen gamma chain is expressed as two isoforms that result from alternative mRNA splicing, the γA chain and the γ' chain. Research studies have shown that the fibrinogen gamma chain plays important roles in shaping the architecture of fibrin clots and modulates the innate immune response through interaction with its receptor, CD11b, expressed on neutrophils and monocytes (9,10).
- Chapin, J.C. and Hajjar, K.A. (2015) Blood Rev 29, 17-24.
- Dalmon, J. et al. (1993) Mol Cell Biol 13, 1183-93.
- Huber, P. et al. (1990) J Biol Chem 265, 5695-701.
- Lord, S.T. (2011) Arterioscler Thromb Vasc Biol 31, 494-9.
- Yang, Z. et al. (2000) Proc Natl Acad Sci U S A 97, 14156-61.
- Cesarman-Maus, G. and Hajjar, K.A. (2005) Br J Haematol 129, 307-21.
- Fernandez, P.M. et al. (2004) Semin Thromb Hemost 30, 31-44.
- Merad, M. and Martin, J.C. (2020) Nat Rev Immunol 20, 355-362.
- Yakovlev, S. et al. (2000) Biochemistry 39, 15721-9.
- Siebenlist, K.R. and Mosesson, M.W. (1994) J Biol Chem 269, 28414-9.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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