WB, IP
H M
Endogenous
55
Rabbit IgG
#Q14318
23770
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu72 of human FKBP8 protein.
Background
FKBP8, also known as FKBP38, is a member of the FK506-binding protein (FKBP) family that interacts with the immunosuppressive drug FK506 through a conserved peptidyl-prolyl cis-trans isomerase (PPIase) domain (1,2). In contrast to other FKBP family members, the PPIase activity of FKBP8 is dependent on Ca2+-signaling through a Ca2+-calmodulin binding domain (3). Loss of FKBP8 leads to defects in neural tube and eye development (4,5). FKBP family members generally function as molecular chaperones interacting with distinct proteins (2). FKBP8 is predominantly localized to the endoplasmic reticulum and the mitochondrial outer membrane and has been found to interact with proteins controlling apoptosis, including Bcl-2 and Bcl-xL (6). In addition, FKBP8 was found to promote autophagy through association with Beclin-1, a key component for activation of the VPS34 lipid kinase required for initiation of autophagy (7). FKBP8 also promotes autophagy by inhibiting mTOR, a kinase that plays a key role in cell growth (8). FKBP8 can also play a role in the clearance of damaged mitochondria through mitophagy. It contains an amino-terminal LC3-interacting region (LIR) motif that binds strongly to LC3A and recruits lipidated LC3A to damaged mitochondria to induce Parkin-independent mitophagy and promote mitochondrial fragmentation (9,10).
- Shirane-Kitsuji, M. and Nakayama, K.I. (2014) Int J Biochem Cell Biol 51, 19-22.
- Kang, C.B. et al. (2008) Neurosignals 16, 318-25.
- Edlich, F. et al. (2005) EMBO J 24, 2688-99.
- Wong, R.L. et al. (2008) Hum Mol Genet 17, 587-601.
- Bulgakov, O.V. et al. (2004) Development 131, 2149-59.
- Shirane, M. and Nakayama, K.I. (2003) Nat Cell Biol 5, 28-37.
- Aguilera, M.O. et al. (2022) Biochim Biophys Acta Mol Cell Res 1869, 119212.
- Bai, X. et al. (2007) Science 318, 977-80.
- Bhujabal, Z. et al. (2017) EMBO Rep 18, 947-961.
- Yoo, S.M. et al. (2020) FASEB J 34, 2944-2957.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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