WB, IP
H M
Endogenous
52
Rabbit IgG
#Q01543
2313
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Rat, Zebrafish
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val64 of human FLI1 protein.
Background
Friend leukemia integration 1 (FLI1) transcription factor is an ETS domain-containing transcription factor that plays an important and highly conserved role in vertebrate development, particularly hematopoiesis, where it functions to activate transcription of genes that promote erythroblast proliferation (1-4). In mice, the Fli1 locus is a common retroviral insertion site for the Friend murine leukemia virus (F-MuLV), such that a majority of F-MuLV-induced erythroleukemias are associated with aberrant Fli1 expression (5). Notably in humans, aberrant FLI1 expression has also been linked to poor prognosis in acute myeloid leukemia (6). Also in humans, a t(11;22)(q24;q12) chromosomal translocation has been described that generates a chimeric protein (EWS/FLI1) comprised of the amino-terminal transactivation domain of Ewing's sarcoma breakpoint region 1 (EWS) and the carboxy-terminal ETS domain of FLI1 (7). The EWS/FLI1 fusion protein functions as a transcriptional activator that is reportedly responsible for >85% of the known cases of pediatric Ewing’s sarcoma, an aggressive bone and soft tissue tumor (8,9).
- Spyropoulos, D.D. et al. (2000) Mol Cell Biol 20, 5643-52.
- Pimanda, J.E. et al. (2007) Proc Natl Acad Sci U S A 104, 17692-7.
- Jagadeeswaran, P. et al. (2010) Blood Cells Mol Dis 44, 175-80.
- Tijssen, M.R. et al. (2011) Dev Cell 20, 597-609.
- Ben-David, Y. et al. (1990) Proc Natl Acad Sci U S A 87, 1332-6.
- Kornblau, S.M. et al. (2011) Blood 118, 5604-12.
- May, W.A. et al. (1993) Proc Natl Acad Sci U S A 90, 5752-6.
- Braun, B.S. et al. (1995) Mol Cell Biol 15, 4623-30.
- Riggi, N. and Stamenkovic, I. (2007) Cancer Lett 254, 1-10.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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