WB
H M R Mk
Endogenous
49
Rabbit
#O75955
10211
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Ile368 of human flotillin-1. Antibodies are purified by peptide affinity chromatography.
Background
Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry an evolutionarily conserved domain called the prohibitin homology domain (PHB) (1). Flotillin members are ubiquitously expressed and located in noncaveolar microdomains (lipid rafts) on the plasma membrane where they support signal transduction and regulate lipid raft motility and localization (2-5). Two flotillin members have been described, flotillin-1 and flotillin-2. In addition to its colocalization with lipid rafts on the plasma membrane, flotillin-1 also has been found in compartments of the endocytic and autophagosomal pathways, such as recycling/late endosomes, the Golgi complex, and the nucleus (6,7). Flotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. However, overexpressed flotillin-2 has also been found in the late endosome (4,8,9). Both flotillin-1 and flotillin-2 are commonly used as lipid raft-associated markers.
- Morrow, I.C. and Parton, R.G. (2005) Traffic 6, 725-40.
- Langhorst, M.F. et al. (2005) Cell Mol Life Sci 62, 2228-40.
- Stuermer, C.A. and Plattner, H. (2005) Biochem Soc Symp , 109-18.
- Fernow, I. et al. (2007) Eur J Cell Biol 86, 345-52.
- Neumann-Giesen, C. et al. (2007) J Cell Sci 120, 395-406.
- Liu, J. et al. (2005) J Biol Chem 280, 16125-34.
- Santamaría, A. et al. (2005) Mol Cell Biol 25, 1900-11.
- Solomon, S. et al. (2002) Immunobiology 205, 108-19.
- Neumann-Giesen, C. et al. (2004) Biochem J 378, 509-18.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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