| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody | 9464 | 20 µl | 78 to 82, 95 kDa | Rabbit |
| Phospho-FoxO1 (Ser256) Antibody | 9461 | 20 µl | 82 kDa | Rabbit |
| Phospho-FoxO1 (Thr24)/FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb | 2599 | 20 µl | 65, 78 to 82, 95 kDa | Rabbit |
| FoxO1 (C29H4) Rabbit mAb | 2880 | 20 µl | 78 to 82 kDa | Rabbit IgG |
| Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb | 13129 | 20 µl | 97 kDa | Rabbit IgG |
| Phospho-FoxO3a (Ser318/321) Antibody | 9465 | 20 µl | 97 kDa | Rabbit |
| FoxO4 Antibody | 9472 | 20 µl | 65 kDa | Rabbit |
| FoxO3a (D19A7) Rabbit mAb | 12829 | 20 µl | 82 to 97 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
This sampler kit provides an economical means to investigate Forkhead signaling. The kit contains primary and secondary antibodies to perform two Western blots with each antibody.
Storage
Background
The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).
- Anderson, M.J. et al. (1998) Genomics 47, 187-99.
- Galili, N. et al. (1993) Nat Genet 5, 230-5.
- Borkhardt, A. et al. (1997) Oncogene 14, 195-202.
- Nakae, J. et al. (1999) J Biol Chem 274, 15982-5.
- Rena, G. et al. (1999) J Biol Chem 274, 17179-83.
- Guo, S. et al. (1999) J Biol Chem 274, 17184-92.
- Seoane, J. et al. (2004) Cell 117, 211-23.
- Arden, K.C. (2004) Mol Cell 14, 416-8.
- Yang, Y. et al. (2005) EMBO J 24, 1021-32.
Background References
Trademarks and Patents
Limited Uses
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