WB, IP
H M R
Endogenous
17
Rabbit IgG
#Q8IVP5
139341
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human FUNDC1 protein.
Background
Mitophagy, a selective form of autophagy targeting damaged mitochondria, is regulated through several protein complexes, including FUNDC1, BNIP3 and BNIP3L/Nix, and PINK1/Parkin (reviewed in 1). FUNDC1 resides on the mitochondrial outer membrane protein and regulates mitochondrial dynamics and hypoxia-induced mitophagy (2-4). It contains an amino-terminal LC3-interacting region (LIR) that is responsible for associating with LC3 and recruiting mitochondria to the autophagosome. The phosphorylation state of FUNDC1 can regulate its activity by affecting its binding to LC3 (4-6). Under normal conditions, FUNDC1 is phosphorylated at Tyr18 by Src, which is inhibited during hypoxia, leading to dephosphorylation (4). FUNDC1 activity is also inhibited by phosphorylation by CK2 at Ser13, which is overcome during hypoxia by the phosphatase PGAM5 which dephosphorylates this site (5). Furthermore, the autophagy kinase ULK1 phosphorylates FUNDC1 at Ser17 which enhances binding to LC3 (6). The levels of FUNDC1 can also be tightly regulated through the ubiquitin/proteasome pathway via the E3 ligase MARCH5, which targets FUNDC1 for degradation (7).
- Pickles, S. et al. (2018) Curr Biol 28, R170-R185.
- Chen, M. et al. (2016) Autophagy 12, 689-702.
- Wu, W. et al. (2016) Autophagy 12, 1675-6.
- Liu, L. et al. (2012) Nat Cell Biol 14, 177-85.
- Chen, G. et al. (2014) Mol Cell 54, 362-77.
- Wu, W. et al. (2014) EMBO Rep 15, 566-75.
- Chen, Z. et al. (2017) EMBO Rep 18, 495-509.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
