WB, IP, IF-IC
H M R Mk
Endogenous
60, 70
Rabbit
#Q9UN86
9908
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human G3BP2 protein. Antibodies are purified by peptide affinity chromatography.
Background
Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation (2). As their name suggests, G3BPs were originally identified as proteins that interact with the SH3 domain of Ras GTPase-activating protein (RasGAP) (3). However, a recent study argues that G3BP1 may not be a genuine RasGAP-binding partner, suggesting that further studies are necessary to validate this particular function (4). Rather, G3BPs are well known to be involved in mRNA decay and inhibition of translation initiation (5,6). The SARS-CoV-2 virus has also been reported to target G3BPs via interaction between the viral N protein and the N-terminal domain of G3BP. This binding prevents G3BPs from interacting with other SG-associated proteins, therefore suppressing SG assembly and promoting viral replication (7). G3BP2 in particular has also been reported to play a role in the initiation of breast tumor formation, as well as affect the expression of PD-L1 in breast and glioblastoma cells under stress conditions (8,9).
- Riggs, C.L. et al. (2020) J Cell Sci 133, jcs242487. doi: 10.1242/jcs.242487.
- Wang, F. et al. (2020) Pharmacol Res 161, 105143.
- Parker, F. et al. (1996) Mol Cell Biol 16, 2561-9.
- Annibaldi, A. et al. (2011) PLoS One 6, e29024.
- Fischer-Kešo, R. et al. (2014) Mol Cell Biol 34, 4244-56.
- Fischer, J.W. et al. (2020) Mol Cell 78, 70-84.e6.
- Luo, L. et al. (2021) Sci Bull (Beijing) 66, 1194-1204.
- Gupta, N. et al. (2017) Proc Natl Acad Sci U S A 114, 1033-1038.
- Zhang, Y. et al. (2021) Mol Oncol, doi: 10.1002/1878-0261.12915.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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