WB
H M R
Endogenous
90
Rabbit
#Q9UQC2
9846
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Gab2 protein. Antibodies are purified by peptide affinity chromatography.
Background
GRB2-associated-binding protein 2 (Gab2) belongs to the Gab family of signaling scaffolds, which are characterized by a highly conserved N-terminal PH domain for membrane recruitment, multiple proline-rich motifs for the recruitment of SH3 domain-containing proteins, such as GRB2, and multiple tyrosine residues capable of associating with SH2 domain-containing proteins, such as p85 PI3K, CrkL, and SHP-2, in a phosphorylation-dependent manner (1). Research studies have shown that Gab2 is a widely expressed protein that participates in orchestrating signals downstream of many transmembrane receptors for growth factors and cytokines (2). Indeed, Gab2 was initially identified as a tyrosine-phosphorylated protein associated with SHP-2 and the p85 subunit of PI3K in response to engagement of the IL-3 and M-CSF receptors (3,4). Subsequent studies also identified Gab2 as a participant in signaling cascades triggered by the T cell antigen receptor (5,6). It is now well established that Gab2 is a critical component of signaling cascades triggered by numerous oncogenic tyrosine kinases. In the context of human disease, Gab2 has been shown to be overexpressed in breast cancer and ovarian cancer, which facilitates tumor cell proliferation and survival (7,8).
- Liu, Y. and Rohrschneider, L.R. (2002) FEBS Lett 515, 1-7.
- Gu, H. et al. (1998) Mol Cell 2, 729-40.
- Carlberg, K. and Rohrschneider, L.R. (1997) J Biol Chem 272, 15943-50.
- Gu, H. et al. (1997) J Biol Chem 272, 16421-30.
- Nishida, K. et al. (1999) Blood 93, 1809-16.
- Yamasaki, S. et al. (2001) J Biol Chem 276, 45175-83.
- Dunn, G.P. et al. (2014) Proc Natl Acad Sci U S A 111, 1102-7.
- Bocanegra, M. et al. (2010) Oncogene 29, 774-9.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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