WB, IP
H M
Recombinant and Transfected
Precursor 28 - Mature 13-16
Rabbit IgG
#P39905
2668
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu200 of human GDNF protein.
Background
Glial cell-derived neurotrophic factor (GDNF) plays an important role in the development and maintenance of the central and peripheral nervous system, renal morphogenesis, and spermatogenesis (1). This glycosylated, disulfide-bonded homodimer is a member of the TGF-β superfamily and plays an important role in neuronal survival (2). GDNF and the related GDNF family of ligands (GFLs) neurturin, persephin, and artemin bind to the GDNF family receptor α (GFRα) proteins that signal through the Ret receptor tyrosine kinase (3,4). The effect that GDNF has on degenerating dopamine neurons makes it an important growth factor when studying Parkinson’s disease (PD) (5). GDNF has also been identified as a therapeutic target for amyotrophic lateral sclerosis (ALS), is a suggested biomarker for Alzheimer’s Disease (AD), and has been associated with cancer progression (6-9).
- Fielder, G.C. et al. (2018) Neoplasia 20, 99-117.
- Choi-Lundberg, D.L. et al. (1997) Science 275, 838-41.
- Sariola, H. and Saarma, M. (2003) J Cell Sci 116, 3855-62.
- Tallini, G. and Asa, S.L. (2001) Adv Anat Pathol 8, 345-54.
- Konishi, Y. et al. (2014) J Neurosci 34, 13127-38.
- Cintrón-Colón, A.F. et al. (2020) Cell Tissue Res 382, 47-56.
- Sharif, M. et al. (2021) Neurol Sci 42, 2865-2872.
- Choi, Y.D. et al. (2020) Int J Mol Sci 21, 3586. doi: 10.3390/ijms21103586.
- Yu, Z. et al. (2022) Int J Oncol 61, 109. doi: 10.3892/ijo.2022.5399.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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