WB
H
Endogenous
28
Rabbit IgG
#P13284
10437
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly111 of human GILT protein.
Background
Gamma-interferon-inducible lysosomal thiol reductase (GILT) is synthesized as a precursor glycoprotein, which is proteolytically processed intracellularly into a mature lysosomal thiol oxidoreductase. Research studies have shown that GILT participates in protein thiol reduction within the endosomal pathway and may contribute to shaping the repertoire of epitopes that undergo MHC class II-dependent presentation (1-3). Additional studies have demonstrated that GILT serves as a restriction factor for multiple RNA-based viruses by inhibiting viral entry (4). Given the role of antigen presentation in facilitating an anti-tumor immune response, research studies have suggested that elevated GILT expression in multiple types of human cancers serves as a biomarker for improved survival and response to immune checkpoint blockade (5-8).
- Phan, U.T. et al. (2000) J Biol Chem 275, 25907-14.
- Luster, A.D. et al. (1988) J Biol Chem 263, 12036-43.
- Hastings, K.T. (2013) Front Immunol 4, 429.
- Chen, D. et al. (2019) Emerg Microbes Infect 8, 1511-1523.
- Adams, A.C. et al. (2022) Cancers (Basel) 14, 2200. doi: 10.3390/cancers14092200.
- Hathaway-Schrader, J.D. et al. (2022) Int J Mol Sci 23, 1066. doi: 10.3390/ijms23031066.
- Buetow, K.H. et al. (2019) J Immunol 203, 2577-2587.
- Phipps-Yonas, H. et al. (2013) Front Immunol 4, 425.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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