| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| PKM2 (D78A4) XP® Rabbit mAb | 4053 | 20 µl | 60 kDa | Rabbit IgG |
| GAPDH (D16H11) XP® Rabbit mAb | 5174 | 20 µl | 37 kDa | Rabbit IgG |
| Pyruvate Dehydrogenase (C54G1) Rabbit mAb | 3205 | 20 µl | 43 kDa | Rabbit IgG |
| Hexokinase I (C35C4) Rabbit mAb | 2024 | 20 µl | 102 kDa | Rabbit IgG |
| Hexokinase II (C64G5) Rabbit mAb | 2867 | 20 µl | 102 kDa | Rabbit |
| LDHA (C4B5) Rabbit mAb | 3582 | 20 µl | 37 kDa | Rabbit IgG |
| PKM1/2 (C103A3) Rabbit mAb | 3190 | 20 µl | 60 kDa | Rabbit IgG |
| PFKP (D4B2) Rabbit mAb | 8164 | 20 µl | 80 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
The Glycolysis Antibody Sampler Kit provides an economical means to investigate select enzymes involved in glycolysis. The kit contains enough primary antibody to perform two western blot experiments with each primary antibody.
Storage
Background
Glycolysis is the metabolic process by which glucose is converted to pyruvate in a sequence of enzymatic steps. Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg effect) (1). Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate. Platelet-type phosphofructokinase (PFKP) is expressed in various cell types (2). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate (3). Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues. The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (4). Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+ (5). LDHA expression is induced when the oxygen supply is too low for mitochondrial ATP production (6). The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. The reaction of oxidative decarboxylation of pyruvate serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism (7).
- WARBURG, O. (1956) Science 123, 309-14.
- Morrison, N. et al. (1992) Hum Genet 89, 105-6.
- Barber, R.D. et al. (2005) Physiol Genomics 21, 389-95.
- Christofk, H.R. et al. (2008) Nature 452, 230-3.
- Semenza, G.L. et al. (1996) J Biol Chem 271, 32529-37.
- Semenza, G.L. (2007) Biochem J 405, 1-9.
- Strumiło, S. (2005) Acta Biochim Pol 52, 759-64.
Background References
Trademarks and Patents
Limited Uses
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