Revision 1

#8337

Store at -20C

Glycolysis Antibody Sampler Kit

1 Kit

(8 x 20 microliters)

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Quantity Mol. Wt Isotype/Source
PKM2 (D78A4) Rabbit Monoclonal Antibody405320 µl60 kDaRabbit IgG
GAPDH (D16H11) Rabbit Monoclonal Antibody517420 µl37 kDaRabbit IgG
Pyruvate Dehydrogenase (C54G1) Rabbit Monoclonal Antibody320520 µl43 kDaRabbit IgG
Hexokinase I (C35C4) Rabbit Monoclonal Antibody202420 µl102 kDaRabbit IgG
Hexokinase II (C64G5) Rabbit Monoclonal Antibody286720 µl102 kDaRabbit
LDHA (C4B5) Rabbit Monoclonal Antibody358220 µl37 kDaRabbit IgG
PKM1/2 (C103A3) Rabbit Monoclonal Antibody319020 µl60 kDaRabbit IgG
PFKP (D4B2) Rabbit Monoclonal Antibody816420 µl80 kDaRabbit IgG
Anti-rabbit IgG, HRP-linked Antibody7074100 µlGoat

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Glycolysis Antibody Sampler Kit provides an economical means to investigate select enzymes involved in glycolysis. The kit contains enough primary antibody to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Glycolysis is the metabolic process by which glucose is converted to pyruvate in a sequence of enzymatic steps. Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg effect) (1). Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate. Platelet-type phosphofructokinase (PFKP) is expressed in various cell types (2). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate (3). Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues. The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (4). Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+ (5). LDHA expression is induced when the oxygen supply is too low for mitochondrial ATP production (6). The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. The reaction of oxidative decarboxylation of pyruvate serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism (7).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell types using Hexokinase I (C35C4) Rabbit mAb.
undefined Image 1: Glycolysis Antibody Sampler Kit
Flow cytometric analysis of HNT-34 cells (blue, negative) and K-562 cells (green, positive) using Hexokinase I (C35C4) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
undefined Image 2: Glycolysis Antibody Sampler Kit
Western blot analysis of various cell extracts using Hexokinase II (C64G5) Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).
undefined Image 3: Glycolysis Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell types using PKM1/2 (C103A3) Rabbit mAb.
undefined Image 4: Glycolysis Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.
undefined Image 5: Glycolysis Antibody Sampler Kit
Western blot analysis of extracts from various cell types using LDHA (C4B5) Rabbit mAb.
undefined Image 6: Glycolysis Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines and mouse skeletal muscle using PKM2 (D78A4) XP® Rabbit mAb (upper) or GAPDH (14C10) Rabbit mAb #2118.
undefined Image 7: Glycolysis Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP® Rabbit mAb.
undefined Image 8: Glycolysis Antibody Sampler Kit
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
undefined Image 9: Glycolysis Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using PFKP (D4B2) Rabbit mAb.
undefined Image 10: Glycolysis Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Hexokinase I (C35C4) Rabbit mAb.
undefined Image 11: Glycolysis Antibody Sampler Kit
Confocal immunofluorescent analysis of HeLa cells using PKM1/2 (C103A3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
undefined Image 12: Glycolysis Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.
undefined Image 13: Glycolysis Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using LDHA (C4B5) Rabbit mAb.
undefined Image 14: Glycolysis Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PKM2 (D78A4) XP® Rabbit mAb.
undefined Image 15: Glycolysis Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GAPDH (D16H11) XP® Rabbit mAb.
undefined Image 16: Glycolysis Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Hexokinase I (C35C4) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
undefined Image 17: Glycolysis Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.
undefined Image 18: Glycolysis Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of MCF-7 cells using LDHA (C4B5) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
undefined Image 19: Glycolysis Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human lymphoma using PKM2 (D78A4) XP® Rabbit mAb.
undefined Image 20: Glycolysis Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded mouse colon using GAPDH (D16H11) XP® Rabbit mAb.
undefined Image 21: Glycolysis Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of HeLa cells using Hexokinase I (C35C4) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
undefined Image 22: Glycolysis Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Pyruvate Dehydrogenase (C54G1) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
undefined Image 23: Glycolysis Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using PKM2 (D78A4) XP® Rabbit mAb. Note the lack of staining in the skeletal muscle cells which do not express PKM2 while vessels within the tissue stain positively.
undefined Image 24: Glycolysis Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of C2C12 cells using GAPDH (D16H11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
undefined Image 25: Glycolysis Antibody Sampler Kit
Confocal immunofluorescent analysis of fixed frozen mouse colon labeled with Hexokinase I (C35C4) Rabbit mAb (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with Ras (E4K9L) Rabbit mAb (Alexa Fluor® 647 Conjugate) #37182 (right, red), and DAPI #4083 (right, blue).
undefined Image 26: Glycolysis Antibody Sampler Kit
Confocal immunofluorescent analysis of A204 cells using PKM2 (D78A4) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
undefined Image 27: Glycolysis Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of fixed frozen mouse pancreas labeled with Hexokinase I (C35C4) Rabbit mAb (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with Ras (E4K9L) Rabbit mAb (Alexa Fluor® 647 Conjugate) #37182 (right, red), and DAPI #4083 (right, blue).
undefined Image 28: Glycolysis Antibody Sampler Kit
Flow cytometric analysis of 293T cells, transfected with PKM2 siRNA (blue) or mock transfected (green), using PKM2 (D78A4) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) #8885 was used as a secondary antibody.
undefined Image 29: Glycolysis Antibody Sampler Kit
Confocal immunofluorescent analysis of fixed frozen mouse kidney labeled with Hexokinase I (C35C4) Rabbit mAb (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with Ras (E4K9L) Rabbit mAb (Alexa Fluor® 647 Conjugate) #37182 (right, red), and DAPI #4083 (right, blue).
undefined Image 30: Glycolysis Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Flow cytometric analysis of NIH/3T3 cells using Hexokinase I (C35C4) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
undefined Image 31: Glycolysis Antibody Sampler Kit

Simple Western™ analysis of lysates (0.1 mg/mL) from Cos-7 cells using Hexokinase II (C64G5) Rabbit mAb #2867. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

undefined Image 32: Glycolysis Antibody Sampler Kit
Flow cytometric analysis of Caki-1 cells using LDHA (C4B5) Rabbit mAb (solid line) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
undefined Image 33: Glycolysis Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Simple Western™ analysis of lysates (0.1 mg/mL) from HeLa cells using GAPDH (D16H11) XP ® Rabbit mAb #5174. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
undefined Image 34: Glycolysis Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.