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8337
Glycolysis Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Glycolysis Antibody Sampler Kit #8337

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Glycolysis Antibody Sampler Kit: Image 1

Flow cytometric analysis of 293T cells, transfected with PKM2 siRNA (blue) or mock transfected (green), using PKM2 (D78A4) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) #8885 was used as a secondary antibody.

Glycolysis Antibody Sampler Kit: Image 2

Confocal immunofluorescent analysis of C2C12 cells using GAPDH (D16H11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Glycolysis Antibody Sampler Kit: Image 3

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.

Glycolysis Antibody Sampler Kit: Image 4

Confocal immunofluorescent analysis of HeLa cells using Hexokinase I (C35C4) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Glycolysis Antibody Sampler Kit: Image 5

Western blot analysis of various cell extracts using Hexokinase II (C64G5) Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).

Glycolysis Antibody Sampler Kit: Image 6

Confocal immunofluorescent analysis of MCF-7 cells using LDHA (C4B5) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Glycolysis Antibody Sampler Kit: Image 7

Confocal immunofluorescent analysis of HeLa cells using PKM1/2 (C103A3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

Glycolysis Antibody Sampler Kit: Image 8

Western blot analysis of extracts from various cell lines using PFKP (D4B2) Rabbit mAb.

Glycolysis Antibody Sampler Kit: Image 9

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Glycolysis Antibody Sampler Kit: Image 10

Confocal immunofluorescent analysis of A204 cells using PKM2 (D78A4) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Glycolysis Antibody Sampler Kit: Image 11

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GAPDH (D16H11) XP® Rabbit mAb.

Glycolysis Antibody Sampler Kit: Image 12

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Pyruvate Dehydrogenase (C54G1) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Glycolysis Antibody Sampler Kit: Image 13

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Hexokinase I (C35C4) Rabbit mAb.

Glycolysis Antibody Sampler Kit: Image 14

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using LDHA (C4B5) Rabbit mAb.

Glycolysis Antibody Sampler Kit: Image 15

Western blot analysis of extracts from various cell types using PKM1/2 (C103A3) Rabbit mAb.

Glycolysis Antibody Sampler Kit: Image 16

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PKM2 (D78A4) XP® Rabbit mAb.

Glycolysis Antibody Sampler Kit: Image 17

Immunohistochemical analysis of paraffin-embedded mouse colon using GAPDH (D16H11) XP® Rabbit mAb.

Glycolysis Antibody Sampler Kit: Image 18

Western blot analysis of extracts from various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.

Glycolysis Antibody Sampler Kit: Image 19

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Hexokinase I (C35C4) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Glycolysis Antibody Sampler Kit: Image 20

Western blot analysis of extracts from various cell types using LDHA (C4B5) Rabbit mAb.

Glycolysis Antibody Sampler Kit: Image 21

Immunohistochemical analysis of paraffin-embedded human lymphoma using PKM2 (D78A4) XP® Rabbit mAb.

Glycolysis Antibody Sampler Kit: Image 22

Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP® Rabbit mAb.

Glycolysis Antibody Sampler Kit: Image 23

Western blot analysis of various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.

Glycolysis Antibody Sampler Kit: Image 24

Western blot analysis of extracts from various cell types using Hexokinase I (C35C4) Rabbit mAb.

Glycolysis Antibody Sampler Kit: Image 25

Immunohistochemical analysis of paraffin-embedded human skeletal muscle using PKM2 (D78A4) XP® Rabbit mAb. Note the lack of staining in the skeletal muscle cells which do not express PKM2 while vessels within the tissue stain positively.

Glycolysis Antibody Sampler Kit: Image 26

Western blot analysis of extracts from various cell lines and mouse skeletal muscle using PKM2 (D78A4) XP® Rabbit mAb (upper) or GAPDH (14C10) Rabbit mAb #2118.

To Purchase # 8337T
Product # Size Price
8337T
1 Kit  (8 x 20 µl) $ 554

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
PKM2 (D78A4) XP® Rabbit mAb 4053 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R Mk 60 Rabbit IgG
GAPDH (D16H11) XP® Rabbit mAb 5174 20 µl
  • WB
  • IHC
  • IF
H M R Mk 37 Rabbit IgG
Pyruvate Dehydrogenase (C54G1) Rabbit mAb 3205 20 µl
  • WB
  • IHC
H M R Mk 43 Rabbit IgG
Hexokinase I (C35C4) Rabbit mAb 2024 20 µl
  • WB
  • IP
  • IHC
  • IF
H M 102 Rabbit IgG
Hexokinase II (C64G5) Rabbit mAb 2867 20 µl
  • WB
H M R Mk 102 Rabbit 
LDHA (C4B5) Rabbit mAb 3582 20 µl
  • WB
  • IHC
  • IF
H Mk 37 Rabbit IgG
PKM1/2 (C103A3) Rabbit mAb 3190 20 µl
  • WB
  • IF
H M R Mk 60 Rabbit IgG
PFKP (D4B2) Rabbit mAb 8164 20 µl
  • WB
  • IP
H Mk 80 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Glycolysis Antibody Sampler Kit provides an economical means to investigate select enzymes involved in glycolysis. The kit contains enough primary antibody to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

GAPDH (D16H11) XP® Rabbit mAb, Hexokinase I (C35C4) Rabbit mAb, Hexokinase II (C64G5) Rabbit mAb, LDHA (C4B5) Rabbit mAb, PKM2 (D78A4) XP® Rabbit mAb, and PFKP (D4B2) Rabbit mAb all detect endogenous levels of their respective total proteins. Pyruvate Dehydrogenase (C54G1) Rabbit mAb detects endogenous levels of total Pyruvate Dehydrogenase α1 subunit. PKM1/2 (C103A3) Rabbit mAb detects endogenous levels of total PKM (including M1 and M2) protein.

Source / Purification

Monoclonal antibodies were produced by immunizing animals with a synthetic peptide corresponding to the respective sequences of human GAPDH, Hexokinase I, Hexokinase II, LDHA, PKM2, Pyruvate Dehydrogenase or PFKP protein.

Background

Glycolysis is the metabolic process by which glucose is converted to pyruvate in a sequence of enzymatic steps. Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg effect) (1). Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate. Platelet-type phosphofructokinase (PFKP) is expressed in various cell types (2). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate (3). Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues. The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (4). Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+ (5). LDHA expression is induced when the oxygen supply is too low for mitochondrial ATP production (6). The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. The reaction of oxidative decarboxylation of pyruvate serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism (7).

  1. WARBURG, O. (1956) Science 123, 309-14.
  2. Morrison, N. et al. (1992) Hum Genet 89, 105-6.
  3. Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
  4. Christofk, H.R. et al. (2008) Nature 452, 230-3.
  5. Semenza, G.L. et al. (1996) J Biol Chem 271, 32529-37.
  6. Semenza, G.L. (2007) Biochem J 405, 1-9.
  7. Strumiło, S. (2005) Acta Biochim Pol 52, 759-64.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.