WB, IHC-Bond, IHC-P
H
Endogenous
70-80
Rabbit IgG
#P15509
1438
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human GM-CSF Receptor α protein.
Background
Granulocyte-macrophage colony-stimulating factor receptor subunit alpha (GM-CSF Receptor α), also known as CSF2RA and CD116, is a type 1 transmembrane protein with low affinity for GM-CSF. GM-CSF Receptor α is primarily expressed on myeloid cells, including granulocytes, monocytes, macrophages, and dendritic cells (1). GM-CSF Receptor α forms a heterodimeric receptor complex with GM-CSF Receptor β, creating the high affinity GM-CSF receptor (2). Upon dimerization and ligand binding, the β subunit becomes phosphorylated and transduces signals via Jak/STAT and MAPK pathways for cell proliferation, differentiation, and survival (3,4). Oligomerization of the high affinity GM-CSF Receptor α/β complex is required for optimal intracellular signal transduction (5). Soluble GM-CSF Receptor α, either secreted or cleaved from the cell surface, can competitively bind GM-CSF, preventing membrane receptor signaling (1,6,7). Multiple isoforms of GM-CSF Receptor α have been described.
- Barreda, D.R. et al. (2004) Dev Comp Immunol 28, 509-54.
- Hansen, G. et al. (2008) Cell 134, 496-507.
- Doyle, S.E. and Gasson, J.C. (1998) Blood 92, 867-76.
- Lopez, A.F. et al. (2010) IUBMB Life 62, 509-18.
- McClure, B.J. et al. (2003) Blood 101, 1308-15.
- Raines, M.A. et al. (1991) Proc Natl Acad Sci U S A 88, 8203-7.
- Geijsen, N. et al. (2001) Cytokine Growth Factor Rev 12, 19-25.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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