WB, IF-F
M
Endogenous
25
Rabbit IgG
#P08882
14940
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:800 - 1:1600 |
Storage
For a carrier free (BSA and azide free) version of this product see product #10352.
Specificity / Sensitivity
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val190 of mouse Granzyme C protein.
Background
Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). In mice, ten family members have been identified: A, B, C, D, E, F, G, K, M, and N (2). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein (3). Granzyme C is the closest murine homolog of human Granzyme H and is located directly downstream of GzmB (4). Granzyme C has been demonstrated to induce cell death with a similar potency as Granzyme B, and CTLs from mice with reduced expression of Granzyme C and F display diminished cytotoxicity (5). Granzyme C has been identified as a marker for type 1 innate lymphoid cells (ILC1s) exhibiting cytotoxic effector function in addition to ‘helper-like’ function (6). These cells have been shown to regulate antitumor immunity and autoimmunity in mouse models (6).
- Trapani, J.A. (2001) Genome Biol 2, REVIEWS3014.
- Russell, J.H. and Ley, T.J. (2002) Annu Rev Immunol 20, 323-70.
- Lord, S.J. et al. (2003) Immunol Rev 193, 31-8.
- Cai, S.F. et al. (2009) J Immunol 182, 6287-97.
- Getachew, Y. et al. (2008) J Immunol 181, 7810-7.
- Nixon, B.G. et al. (2022) Sci Immunol 7, eabi8642.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-F: Immunofluorescence (Frozen)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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