IF-IC
H
Endogenous
62
Rabbit IgG
#Q14451
2886
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro87 of human GRB7 protein.
Background
GRB7 belongs to the GRB7 family of adaptor proteins that mediate cell surface and cytosolic tyrosine kinases to downstream signaling pathways (1-3). The C-terminal SH2 domain of GRB7 has been shown to be important for its interaction with various upstream molecules such as ERBB receptor tyrosine kinases, FAK, EphB1, and SHC. The protein has an RA, CAM-BD, and PH domain located at the central region (GM region) of its sequences to mediate downstream Ras, PI3K/Akt, ERK, and Calmodulin signaling (3,4). Overexpression of GRB7 promotes cancer proliferation and migration, and disruption of GRB7 function inhibits its effect on cancer growth, invasion, and metastasis (3-5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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