WB
H M
Endogenous
80
Rabbit IgG
#P35626
157
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys454 of human GRK3 protein.
Background
G-protein-coupled receptor kinase 3 (GRK3), also known as beta-adrenergic receptor kinase 2 (beta-ARK2), is a member of the GRK family, which phosphorylates the activated form of G-protein-coupled receptors (GPCRs) and initiates the desensitization process of GPCR (1). GRK3 has been implicated in the phosphorylation of GPCRs, enabling their interaction with beta-arrestin, and facilitating their signaling through ERK1/2 phosphorylation (2). More recently, GRK3 was found to play a critical role in tumor progression through stimulation of angiogenesis; furthermore, GRK3 was found to be overexpressed in human prostate cancer, in particular in metastatic tumors (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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