WB
H M R Mk
Endogenous
100
Rabbit
#P14625
7184
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Met622 of human Grp94 . Antibodies are purified by protein A and peptide affinity chromatography.
Background
Secretory proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. The ER contains a pool of molecular chaperones, including Grp94, to help ensure correct protein folding. Grp94 is a glucose-regulated protein (1) with sequence homology to Hsp90 (2). In addition to its role in helping to facilitate folding of a number of secretory proteins to their correct conformation (3), studies suggest that Grp94 derived from cancer cells also induces anti-tumor immune responses in mouse tumor models (4, 5). One way in which Grp94 promotes tumor immunogenicity is its ability to bind to and present tumor-derived peptides as antigens (6). Furthermore, Grp94 has also been shown to induce maturation of dendritic cells (7). Taken together, Grp94 functions both as a tumor-specific antigen and as an activator of antigen-presenting cells to elicit an anti-cancer immune response (8).
- Lee, A.S. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 4922-4925.
- Sorger, P.K. and Pelham, H.R. (1987) J. Mol. Biol. 194, 341-344.
- Argon, Y. and Simen, B.B. (1999) Semin. Cell Dev. Biol. 10, 495-505.
- Blachere, N.E. et al. (1997) J. Exp. Med. 186, 1315-1322.
- Tamura, Y. et al. (1997) Science 278, 117-120.
- Schild, H. and Rammensee, H.G. (2000) Nat. Immunol. 1, 100-101.
- Singh-Jasuja, H. et al. (2000) Eur. J. Immunol. 30, 2211-2215.
- Nicchitta, C.V. et al. (2004) Cell Stress Chaperones 9, 325-331.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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