WB, IP, IHC-P
H
Endogenous
145, 130
Rabbit IgG
#P25092
2984
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys1046 of human GUCY2C protein.
Background
Guanylyl cyclase C (GUCY2C) is a type I transmembrane glycoprotein whose expression in normal tissue is largely restricted to the apical membranes of polarized epithelial cells of the intestine (1,2). The guanylyl cyclase activity within the intracellular domain of GUCY2C is activated by binding exogenous bacterial enterotoxins and the endogenous peptide hormone agonists, guanylin and uroguanylin, which facilitates production of the second messenger cGMP (3-5). GUCY2C signaling plays a critical role in regulating the metabolism, proliferation, and differentiation of intestinal epithelial cells. These processes are critical for epithelial barrier renewal, water, and ion balance (6-8). Research studies have shown that the GUCY2C signaling axis functions to suppress intestinal tumorigenesis, in part, by restraining AKT activity (9,10). Lesions in the GUCY2C signaling axis that frequently contribute to cellular transformation are associated with a loss of expression of its endogenous ligands, which promotes a compensatory increase in GUCY2C expression. Indeed, GUCY2C expression is maintained in a high percentage of primary and metastatic human colorectal tumors, which has raised interest in therapeutically targeting this receptor (11,12).
- Valentino, M.A. et al. (2011) J Clin Invest 121, 3578-88.
- Kim, G.W. et al. (2013) Trends Endocrinol Metab 24, 165-73.
- Currie, M.G. et al. (1992) Proc Natl Acad Sci U S A 89, 947-51.
- Kita, T. et al. (1994) Am J Physiol 266, F342-8.
- Schulz, S. et al. (1990) Cell 63, 941-8.
- Li, P. et al. (2007) Am J Pathol 171, 1847-58.
- Li, P. et al. (2007) Gastroenterology 133, 599-607.
- Pitari, G.M. et al. (2001) Proc Natl Acad Sci U S A 98, 7846-51.
- Lin, J.E. et al. (2010) Gastroenterology 138, 241-54.
- Gibbons, A.V. et al. (2013) Cancer Res 73, 6654-66.
- Mathur, D. et al. (2020) Clin Cancer Res 26, 2188-2202.
- Magee, M.S. et al. (2018) Cancer Immunol Res 6, 509-516.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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