WB
H M R Mk
Endogenous
49
Rabbit
#O14929
8520
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val412 of human HAT1 protein. Antibodies are purified by peptide affinity chromatography.
Background
Histone acetyltransferase 1 (HAT1) is a member of the GCN5 family of acetyltransferases that can acetylate Lys5 and Lys12 on newly generated histone H4, a process that is essential for mammalian development (1-5). HAT1 binds to the H4 gene promoter, where it facilitates the production and acetylation of new histones. This process is strongly dependent on glucose availability, suggesting HAT1 plays a role in nutrient sensing (6). HAT1 has also been shown to contribute to recovery from replication-based DNA damage by incorporating H4Lys5/Lys12 acetylated histones at the sites of double-stranded breaks (7-9). HAT1 can acetylate non-histone proteins as well, and has been shown to play a key role in NF-kB signaling by acetylating PLZF, which in turn forms a complex with p50 to limit the production of cytokines (10). HAT1 overexpression has also been implicated in many cancer types (11-13).
- Kleff, S. et al. (1995) J Biol Chem 270, 24674-7.
- Verreault, A. et al. (1998) Curr Biol 8, 96-108.
- Ruiz-García, A.B. et al. (1998) J Biol Chem 273, 12599-605.
- Dutnall, R.N. et al. (1998) Cell 94, 427-38.
- Nagarajan, P. et al. (2013) PLoS Genet 9, e1003518.
- Gruber, J.J. et al. (2019) Mol Cell 75, 711-724.e5.
- Barman, H.K. et al. (2006) Biochem Biophys Res Commun 345, 1547-57.
- Benson, L.J. et al. (2007) J Biol Chem 282, 836-42.
- Yang, X. et al. (2013) J Biol Chem 288, 18271-82.
- Sadler, A.J. et al. (2015) Nat Commun 6, 6795.
- Min, S.K. et al. (2012) Korean J Pathol 46, 142-50.
- Miao, B.P. et al. (2018) Arch Biochem Biophys 646, 72-79.
- Fan, P. et al. (2019) J Exp Clin Cancer Res 38, 47.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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