WB
H R
Endogenous
135, 155
Rabbit IgG
#Q12809
3757
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Rat
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala223 of human hERG1a protein.
Background
hERG1(human ether-a-go-go-related gene potassium channel 1) is a voltage gated potassium channel alpha-subunit which mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr) (1,2). The hERG channel is composed of two subunits, 1a and 1b, which differ at amino terminus due to alternative splicing. Native hERG channels are heteromers of hERG1a with hERG1b. Both subunits contribute to IKr current (3-6).
Blockade of hERG currents induced by compounds or mutation of hERG encoding gene-KCNH2 causes ventricular arrhythmias associated with inherited and acquired long QT syndrome and cardiomyocyte apoptosis (7-10). Therefore, hERG channel is a primary target for the development of class III antiarrhythmic agents (11,12). The hERG channel is also inhibited by a variety of non-antiarrhythmic compounds, which result in side effects. Consequently, hERG channel blockage is a common counter screen when selecting therapeutic agents for various diseases (11,13,14).
Research studies have implicated hERG in cancer cell survival (15). In normal human adult tissue, hERG is expressed in heart, brain, myometrium, pancreas, and hematopoietic progenitors (16,17). hERG is expressed in various cancer cell lines of epithelial, neuronal, leukemic, and connective tissue origin but not in corresponding normal cells (18-22). Furthermore, hERG expression is associated with enhanced cancer cell proliferation, invasiveness, and poor prognosis (23,24).
- Warmke, J.W. and Ganetzky, B. (1994) Proc Natl Acad Sci U S A 91, 3438-42.
- Sanguinetti, M.C. and Tristani-Firouzi, M. (2006) Nature 440, 463-9.
- Lees-Miller, J.P. et al. (1997) Circ Res 81, 719-26.
- London, B. et al. (1997) Circ Res 81, 870-8.
- Lees-Miller, J.P. et al. (2003) Mol Cell Biol 23, 1856-62.
- Sale, H. et al. (2008) Circ Res 103, e81-95.
- Curran, M.E. et al. (1995) Cell 80, 795-803.
- Itoh, T. et al. (1998) Hum Genet 102, 435-9.
- González-Juanatey, J.R. et al. (2003) Circulation 107, 127-31.
- Gong, Q. et al. (2006) J Biol Chem 281, 4069-74.
- Thomas, D. et al. (2006) Curr Pharm Des 12, 2271-83.
- Staudacher, I. et al. (2010) Curr Opin Drug Discov Devel 13, 23-30.
- Wible, B.A. et al. (2005) J Pharmacol Toxicol Methods 52, 136-45.
- Yang, B.F. et al. (2004) Acta Pharmacol Sin 25, 554-60.
- Jehle, J. et al. (2011) Cell Death Dis 2, e193.
- Pond, A.L. et al. (2000) J Biol Chem 275, 5997-6006.
- Rosati, B. et al. (2000) FASEB J 14, 2601-10.
- Smith, G.A. et al. (2002) J Biol Chem 277, 18528-34.
- Lastraioli, E. et al. (2004) Cancer Res 64, 606-11.
- Masi, A. et al. (2005) Br J Cancer 93, 781-92.
- Lin, H. et al. (2007) J Cell Physiol 212, 137-47.
- Gong, J.H. et al. (2010) Oncol Rep 23, 1747-56.
- Ding, X.W. et al. (2008) J Surg Oncol 97, 57-62.
- Shao, X.D. et al. (2008) Cancer Biol Ther 7, 45-50.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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