WB
H
Endogenous
90
Rabbit IgG
#Q8NCD3
55355
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly672 of human HJURP protein.
Background
CENP-A is an essential histone H3 variant that replaces canonical histone H3 in centromeric heterochromatin. The inherited localization of the centromere is specified by CENP-A (1). CENP-A deposition to the correct chromosomal location in early G1 phase is regulated by the Mis18 complex, which recruits the CENP-A assembly factor/chaperone protein HJURP (Holliday Junction Recognition Protein) (2-3).
Dimerization of HJURP is required for its activity (4), and phosphorylation by cyclin dependent kinases is required for temporal regulation of HJURP recruitment (5).
Overexpression of HJURP causes chromosome loss in yeast and mitotic defects in mammalian cells (6). Further, downregulation of HJURP expression has been associated with replicative senescence in human cells (7).
Research studies indicate that HJURP may have prognostic value in human breast cancer and high grade gliomas (8-10).
- Ausió, J. (2006) Brief Funct Genomic Proteomic 5, 228-43.
- Stellfox, M.E. et al. (2013) Cell Mol Life Sci 70, 387-406.
- Wang, J. et al. (2014) J Biol Chem 289, 8326-36.
- Zasadzińska, E. et al. (2013) EMBO J 32, 2113-24.
- Müller, S. et al. (2014) Cell Rep 8, 190-203.
- Mishra, P.K. et al. (2011) PLoS Genet 7, e1002303.
- Heo, J.I. et al. (2013) J Gerontol A Biol Sci Med Sci 68, 914-25.
- Hu, Z. et al. (2010) Breast Cancer Res 12, R18.
- Montes de Oca, R. et al. (2015) Mol Oncol 9, 657-74.
- de Tayrac, M. et al. (2013) PLoS One 8, e73332.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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