Revision 1

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Quantity Mol. Wt Isotype/Source
ATM (D2E2) Rabbit mAb287320 µl350 kDaRabbit IgG
Phospho-ATM (Ser1981) (D25E5) Rabbit mAb1305020 µl350 kDaRabbit IgG
Rad51 (D4B10) Rabbit mAb887520 µl37 kDaRabbit IgG
BRCA1 (A8X9F) Rabbit mAb1482320 µl220 kDaRabbit IgG
BRCA2 (D9S6V) Rabbit mAb1074120 µl380 kDaRabbit IgG
Rad54 (D4W3Z) Rabbit mAb1501620 µl84 kDaRabbit IgG
p95/NBS1 (D6J5I) Rabbit mAb1495620 µl95 kDaRabbit IgG
Phospho-p95/NBS1 (Ser343) Antibody300120 µl95 kDaRabbit
CtIP (D76F7) Rabbit mAb920120 µl110 kDaRabbit IgG
Anti-rabbit IgG, HRP-linked Antibody7074100 µlGoat

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Homologous Recombination (HR) DNA Repair Antibody Sampler Kit provides an economical means of detecting proteins involved in HR DNA repair. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells recognize and repair DSBs via two distinct but partly overlapping signaling pathways, non-homologous end joining (NHEJ) and homologous recombination (HR). DSBs that arise during S or G2 phase are repaired via HR, using the replicated sister chromatid as a repair template (1). Activation of ATM by autophosphorylation on Ser1981 occurs in response to exposed DNA DSBs. ATM regulates various responses to DNA damage, including regulation of HR factors (2). Rad51 recombinase polymerizes and forms a filament along single-stranded DNA, mediating HR with the help of auxiliary proteins, including Rad54 and BRCA2 (3). BRCA2 has been shown to be required for localization of Rad51 to sites of DSBs, and cells lacking BRCA1 and BRCA2 cannot repair DSBs through HR (4). NBS1 is critical for HR, and requires CDK-dependent association with CtIP and subsequent phosphorylation by ATM at Ser278 and Ser343 (5-6).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using BRCA2 (D9S6V) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
undefined Image 1: Homologous Recombination (HR) DNA Repair Antibody Sampler Kit
Western blot analysis of extracts from HCT 116 cells, untreated (-) or treated with neocarzinostatin (NCS 10 μM, 1 hr; +), using Phospho-ATM (Ser1981) (D25E5) Rabbit mAb (upper) and ATM (D2E2) Rabbit mAb #2873 (lower).
undefined Image 2: Homologous Recombination (HR) DNA Repair Antibody Sampler Kit
Western blot analysis of extracts from HT-29, HeLa, and MCF7 cells using BRCA1 (A8X9F) Rabbit mAb.
undefined Image 3: Homologous Recombination (HR) DNA Repair Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using p95/NBS1 (D6J5I) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
undefined Image 4: Homologous Recombination (HR) DNA Repair Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using Rad54 (D4W3Z) Rabbit mAb.
undefined Image 5: Homologous Recombination (HR) DNA Repair Antibody Sampler Kit
Western blot analysis of extracts of HeLa, NCCIT and PYS2 cells using ATM (D2E2) Rabbit mAb.
undefined Image 6: Homologous Recombination (HR) DNA Repair Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from 293 cells, untreated or UV-treated (100 mJ/cm2, 1 hr), using Phospho-p95/NBS1 (Ser343) Antibody (upper) or p95/NBS1 (E8M3Q) XP® Rabbit mAb #81234 (lower).
undefined Image 7: Homologous Recombination (HR) DNA Repair Antibody Sampler Kit
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
undefined Image 8: Homologous Recombination (HR) DNA Repair Antibody Sampler Kit
Western blot analysis of extracts from various cell lines using Rad51 (D4B10) Rabbit mAb.
undefined Image 9: Homologous Recombination (HR) DNA Repair Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using CtIP (D76F7) Rabbit mAb.
undefined Image 10: Homologous Recombination (HR) DNA Repair Antibody Sampler Kit
Western blot analysis of DLD1 cells, either wild type (WT) or BRCA2 knockout (-/-), using BRCA2 (D9S6V) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb (lower). DLD1 (WT) and DLD1 (-/-) cells were kindly provided by Dr. Judit Jimenez, Yale University, New Haven, CT.
undefined Image 11: Homologous Recombination (HR) DNA Repair Antibody Sampler Kit
Immunoprecipitation of p95/NBS1 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or p95/NBS1 (D6J5I) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using p95/NBS1 (D6J5I) Rabbit mAb.
undefined Image 12: Homologous Recombination (HR) DNA Repair Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunoprecipitation of Rad54 from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Rad54 (D4W3Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Rad54 (D4W3Z) Rabbit mAb.
undefined Image 13: Homologous Recombination (HR) DNA Repair Antibody Sampler Kit
Immunoprecipitation of Rad51 from HeLa cell extracts using Rad51 (D4B10) Rabbit mAb (lane 2). Western blot detection was performed using the same antibody. Lane 1 is 10% input.
undefined Image 14: Homologous Recombination (HR) DNA Repair Antibody Sampler Kit
Confocal immunofluorescent analysis of SK-MEL-28 (left) and GM07166 (right) cells using p95/NBS1 (D6J5I) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054.
undefined Image 15: Homologous Recombination (HR) DNA Repair Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo

Simple Western™ analysis of lysates (0.1 mg/mL) from 293 cells treated with UV (100 mJ/cm2; 2 Hour Recovery) using Phospho-ATM (Ser1981) (D25E5) Rabbit mAb #13050. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 66 - 440 kDa separation module.

undefined Image 16: Homologous Recombination (HR) DNA Repair Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.