WB
H
Endogenous
50, 65
Rabbit IgG
#Q9Y251
10855
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu466 of human HPSE protein.
Background
Heparanase (HPSE) is an endo-β-D-endoglycosidase that catalyzes the cleavage heparan sulfate (HS) side chains from proteoglycans at the cell surface (1). The protein is synthesized as a 65 kDa inactive precursor that undergoes proteolytic processing, yielding 8 kDa and 50 kDa protein subunits that heterodimerize to form an active enzyme (2). HPSE serves as a key modulator of extracellular matrix (ECM) basic structure. The cleavage of HS from proteoglycans and associated changes at the ECM also cause the release of growth factors from the matrix to bind to their cognate receptors, leading to activation of downstream signaling pathways (3). A small percentage of HPSE localizes to specialized lipid raft regions. There, HPSE activates multiple signaling pathways, such as Akt, ERK, and Src pathways, by a nonenzymatic mechanism mediated by its C-terminal domain (4,5). HPSE is involved in the development of diseases such as fibrosis initiation, inflammation, tumor progression, and viral infection (6-9). HPSE inhibitors have been developed and pursued as novel therapeutics for these diseases (10-12).
- Vlodavsky, I. et al. (2020) Adv Exp Med Biol 1221, 3-59.
- Vlodavsky, I. et al. (2007) Curr Pharm Des 13, 2057-73.
- Masola, V. et al. (2018) Cells 7, 236. doi: 10.3390/cells7120236.
- Ben-Zaken, O. et al. (2007) Biochem Biophys Res Commun 361, 829-34.
- Fux, L. et al. (2009) Trends Biochem Sci 34, 511-9.
- Koganti, R. et al. (2020) Cell Mol Life Sci 77, 5059-5077.
- Secchi, M.F. et al. (2015) Biomol Concepts 6, 415-21.
- Thakkar, N. et al. (2017) Pathogens 6, 43. doi: 10.3390/pathogens6030043.
- Masola, V. et al. (2015) J Transl Med 13, 181.
- Simeonovic, C.J. et al. (2013) Front Immunol 4, 471.
- Kaur, R. et al. (2021) Curr Pharm Des 27, 43-68.
- Kaltenbach, D.D. et al. (2018) Front Pharmacol 9, 1315.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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