WB
H
Endogenous
29
Rabbit IgG
#O14756
8630
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu220 of human HSD17B6 protein.
Background
The type 6 17-β-hydroxysteroid dehydrogenase (HSD17B6, 17-β-HSD 6) regulates cellular hydroxysteroids by catalyzing the conversion of androsterone to epiandrosterone (1). This enzyme displays both oxidoreductase and epimerase activities, and is also known as 3(α->β)-hydroxysteroid epimerase. The interaction between HSD17B6 and hydroxysteroid compounds has an important effect on steroid activity as these compounds typically act in a stereo-specificity manner (1). Research studies show that the transcriptional activity of androgen receptor in prostate cell lines treated by androstanediol correlates with HSD17B6 protein level, which suggests an important role for enzyme in prostate cancer growth (2).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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