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62714
Human Immune Cell Phenotyping IHC Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Human Immune Cell Phenotyping IHC Antibody Sampler Kit #62714

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Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 1

Immunohistochemical analysis of paraffin-embedded human tonsil using CD3ε (D7A6E™) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 2

Immunohistochemical analysis of paraffin-embedded human mucoepidermoid carcinoma of the larynx using CD3ε (D7A6E™) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 3

Immunohistochemical analysis of paraffin-embedded human appendix using CD8α (C8/144B) Mouse mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 4

Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma using FoxP3 (D2W8E™) (IHC Specific) Rabbit mAb performed on the Leica® BOND™ Rx.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 5

Immunohistochemical analysis of paraffin-embedded human endometrioid carcinoma using CD11b/ITGAM (D6X1N) Rabbit mAb performed on the Leica® Bond™ Rx.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 6

Flow cytometric analysis of fixed and permeabilized Jurkat cells (blue, negative) and THP-1 cells (green, positive) using CD68 (D4B9C) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 7

Immunohistochemical analysis of paraffin-embedded human tonsil using CD68 (D4B9C) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 8

Confocal immunofluorescent analysis of THP-1 cells, differentiated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (80 nM, 24 hr) followed by 24 hr without TPA, (left, positive) or HeLa cells (right, negative), using CD11c (D3V1E) XP® Rabbit mAb #45581 (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 9

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using CD11c (D3V1E) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 10

Immunohistochemical analysis of paraffin-embedded human endometrioid carcinoma using CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb performed on the Leica® Bond™ Rx.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 11

Flow cytometric analysis of A431 cells using Pan-Keratin (C11) Mouse mAb (solid line) versus a concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed line). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 12

Confocal immunofluorescent analysis of SH-SY5Y cells (left, positive) or HeLa cells (right, negative) using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (red). Cells were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 13

Flow cytometric analysis of HeLa cells (blue) and SH-SY5Y cells (green) using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 14

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 15

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using CD3ε (D7A6E™) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 16

Immunohistochemical analysis of paraffin-embedded human lymph node using CD8α (C8/144B) Mouse mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 17

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using FoxP3 (D2W8E™) Rabbit mAb (IHC Specific).

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 18

Immunohistochemical analysis of paraffin-embedded human clear cell adenocarcinoma of the ovary using CD11b/ITGAM (D6X1N) Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 19

Flow cytometric analysis of live human peripheral blood mononuclear cells co-stained with anti-human CD14 using CD68 (D4B9C) XP® Rabbit mAb (right) compared to a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 20

Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using CD68 (D4B9C) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 21

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 22

Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 23

Confocal immunofluorescent analysis of Neuro-2a cells (left, high-expressing) or C2C12 cells (right, low-expressing) using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (red). Cells were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 24

Confocal immunofluorescent analysis of mouse spinal cord (left), or rat brainstem (right) using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (red). Tissue was mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 25

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 26

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CD3ε (D7A6E™) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 27

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using CD8α (C8/144B) Mouse mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 28

Immunohistochemical analysis of paraffin-embedded COS-7 cell pellets, untransfected (left) or transfected with human FoxP3 protein (right), using FoxP3 (D2W8E™) Rabbit mAb (IHC Specific).

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 29

Immunohistochemical analysis of paraffin-embedded THP-1 cell pellet (left, positive) or Jurkat cell pellet (right, negative) using CD11b/ITGAM (D6X1N) Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 30

Flow cytometric analysis of fixed and permeabilized human peripheral blood mononuclear cells co-stained with anti-human CD14 using CD68 (D4B9C) XP® Rabbit mAb (right) compared to a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 31

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CD68 (D4B9C) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 32

Immunohistochemical analysis of paraffin-embedded THP-1 cell pellet (left, positive) or Raji cell pellet (right, negative) using CD11c (D3V1E) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 33

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 34

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma (bladder), using Pan-Keratin (C11) Mouse mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 35

Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 36

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using CD3ε (D7A6E™) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 37

Immunohistochemical analysis of paraffin-embedded human spleen using CD8α (C8/144B) Mouse mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 38

Immunohistochemical analysis of paraffin-embedded human tonsil using FoxP3 (D2W8E™) Rabbit mAb (IHC Specific).

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 39

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD11b/ITGAM (D6X1N) Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 40

Confocal immunofluorescent analysis of THP-1 (left) and Jurkat (right) cells using CD68 (D4B9C) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Projected images from a z-stack series are shown.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 41

Immunohistochemical analysis of paraffin-embedded THP-1 (left) and Jurkat (right) cell pellets using CD68 (D4B9C) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 42

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD11c (D3V1E) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 43

Immunohistochemical analysis of paraffin-embedded human lymphoma using CD19 (Intraceullular Domain) (D4V4B) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 44

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Pan-Keratin (C11) Mouse mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 45

Immunohistochemical analysis of paraffin-embedded mouse ovary using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 46

Immunohistochemical analysis of paraffin-embedded MOLT-4 (left) and PC-3 (right) cell pellets using CD3ε (D7A6E™) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 47

Western blot analysis of extracts from various cells using CD11b/ITGAM (D6X1N) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 48

Multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using CD68 (D4B9C) XP® rabbit mAb (red), PD-1 (EH33) mouse mAb #43248 (green), CD8α (C8/144B) mouse mAb #70306 (magenta), Pan-keratin (C11) mouse mAb #4545 (cyan), LAG3 (D2G4O™) XP® rabbit mAb #15372 (orange), and TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow).

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 49

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using CD11c (D3V1E) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 50

Immunohistochemical analysis of paraffin-embedded mouse spleen using CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 51

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Pan-Keratin (C11) Mouse mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 52

Immunohistochemical analysis of paraffin-embedded endometrioid adenocarcinoma using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (left) or NCAM (CD56) (123C3) Mouse mAb #3576 (right). These two antibodies detect independent, unique epitopes on human NCAM1. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 53

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma with staining of peripheral nerve using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 54

Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using CD68 (D4B9C) XP® rabbit mAb (orange), PD-L1 (E1L3N®) XP® rabbit mAb #13684 (red), PD-L2 (D7U8C™) XP® rabbit mAb (magenta) #82723, Arginase-1 (D4E3M™) XP® rabbit mAb #93668 (green), IDO (D5J4E™) rabbit mAb #86630 (yellow), and Pan-keratin (C11) mouse mAb #4545 (cyan).

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 55

Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using CD11c (D3V1E) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 56

Immunohistochemical analysis of paraffin-embedded human tonsil using CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 57

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Pan-Keratin (C11) Mouse mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 58

Immunohistochemical analysis of paraffin-embedded SH-SY5Y cell pellet (left, positive) or MCF7 cell pellet (right, negative) using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 59

Western blot analysis of extracts from MOLT-4, HuT 102, and PC-3 cells using CD3ε (D7A6E™) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 60

Western blot analysis of extracts from various cell lines using CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, CD19 protein is not detected in either U-937 cells or Raw 264.7 cells.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 61

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Pan-Keratin (C11) Mouse mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 62

Western blot analysis of extracts from THP-1, Raji, or Jurkat cells using CD11c (D3V1E) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 63

Western blot analysis of extracts from Daudi cells, untreated (-) or treated with PNGase F (+), using CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 64

Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Pan-Keratin (C11) mouse mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 65

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Pan-Keratin (C11) mouse mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 66

Immunohistochemical analysis of paraffin-embedded mouse colon using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 67

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Pan-Keratin (C11) mouse mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 68

Western blot analysis of extracts from various cell lines, using Pan-Keratin (C11) Mouse mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 69

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 70

Immunohistochemical analysis of paraffin-embedded mouse prostate using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 71

Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 72

Immunoprecipitation of NCAM1 protein from SH-SY5Y cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is NCAM1 (CD56) (E7X9M) XP® Rabbit mAb. Western blot analysis was performed using NCAM1 (CD56) (123C3) Mouse mAb #3576. Anti-mouse IgG, HRP-linked Antibody #7076 was used as the secondary antibody.

Human Immune Cell Phenotyping IHC Antibody Sampler Kit: Image 73

Western blot analysis of extracts from various cell lines and tissues using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

To Purchase # 62714T
Product # Size Price
62714T
1 Kit  (9 x 20 µl) $ 609

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CD3ε (D7A6E™) XP® Rabbit mAb 85061 20 µl
  • WB
  • IP
  • IHC
H 23 Rabbit IgG
CD8α (C8/144B) Mouse mAb (IHC Specific) 70306 20 µl
  • IHC
H Mouse IgG1
FoxP3 (D2W8E™) Rabbit mAb (IHC Specific) 98377 20 µl
  • IHC
H 45 Rabbit IgG
CD11b/ITGAM (D6X1N) Rabbit mAb 49420 20 µl
  • WB
  • IHC
H 170 Rabbit IgG
CD68 (D4B9C) XP® Rabbit mAb 76437 20 µl
  • IHC
  • IF
  • F
H Rabbit IgG
CD11c (D3V1E) XP® Rabbit mAb 45581 20 µl
  • WB
  • IHC
  • IF
H 145 Rabbit IgG
CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb 90176 20 µl
  • WB
  • IP
  • IHC
H M 95 Rabbit IgG
Pan-Keratin (C11) Mouse mAb 4545 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk 46-58 Mouse IgG1
NCAM1 (CD56) (E7X9M) XP® Rabbit mAb 99746 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R 120 to 220 Rabbit IgG

Product Description

The Human Immune Cell Phenotyping IHC Antibody Sampler Kit provides an economical means of detecting the accumulation of immune cell types in formalin-fixed, paraffin-embedded tissue samples.

Specificity / Sensitivity

Each antibody included in the Human Immune Cell Phenotyping IHC Antibody Sampler Kit recognizes endogenous levels of its target human protein. CD11c (D3V1E) XP® Rabbit mAb does not cross-react with CD11b. Pan-Keratin (C11) Mouse mAb detects endogenous levels of total keratin 4, 5, 6, 8, 10, 13, and 18. Pan-Keratin (C11) Mouse mAb does not cross-react with other keratins.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Glu178 of human CD3ε protein, Leu427 of human CD19 protein, Pro799 of human NCAM1/CD56 protein, near the carboxy terminus of human CD8 protein, or with recombinant protein specific to human FoxP3, human CD68, human CD11c, or the amino terminus of human CD11b/ITGAM protein. Pan-Keratin (C11) Mouse mAb (isotype: IgG1) is produced by immunizing a BALB/c mouse with a cytoskeleton preparation from A431 cells.

Background

Cluster of Differentiation 3 (CD3) is a multiunit protein complex expressed on the surface of T cells that directly associates with the T cell receptor (TCR). CD3 is composed of four polypeptides: ζ, γ, ε and δ. Engagement of the TCR complex with antigens presented in Major Histocompatibility Complexes (MHC) induces tyrosine phosphorylation in the immunoreceptor tyrosine-based activation motif (ITAM) of CD3 proteins. CD3 phosphorylation is required for downstream signaling through ZAP-70 and p85 subunit of PI-3 kinase, leading to T cell activation, proliferation, and effector functions (1). CD8 is a transmembrane glycoprotein expressed primarily on cytotoxic T cells, but has also been described on a subset of dendritic cells in mice (2,3). On T cells, CD8 is a co-receptor for the TCR, and these two distinct structures are required to recognize antigen bound to MHC Class I. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and recruits the tyrosine kinase Lck, which is essential for T cell activation (2). Forkhead box P3 (FoxP3) is crucial for the development of T cells with immunosuppressive regulatory properties and is a well-established marker for CD4+ T regulatory cells (Tregs) (4). Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein forming heterodimers that are composed of α and β subunits (5). CD11b is expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (6). CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (7,8). It is found on the plasma membrane, as well as endosomal and lysosomal membranes (7-9). CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein highly expressed by dendritic cells, and has also been observed on activated NK cells, subsets of B and T cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (10,11). CD19 is a co-receptor expressed on B cells that amplifies the signaling cascade initiated by the B cell receptor (BCR) to induce activation. It is a biomarker of B lymphocyte development, lymphoma diagnosis, and can be utilized as a target for leukemia immunotherapies (12,13). NCAM (neural cell adhesion molecule, CD56) is an adhesion glycoprotein with five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats (14). CD56 and CD16 are commonly used to identify NK cells although some cells with the T cell markers CD3 and CD4 also express CD56 (15). Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (16,17). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (16).

  1. Kuhns, M.S. et al. (2006) Immunity 24, 133-9.
  2. Zamoyska, R. (1994) Immunity 1, 243-6.
  3. Shortman, K. and Heath, W.R. (2010) Immunol Rev 234, 18-31.
  4. Ochs, H.D. et al. (2007) Immunol Res 38, 112-21.
  5. Solovjov, D.A. et al. (2005) J Biol Chem 280, 1336-45.
  6. Murray, P.J. and Wynn, T.A. (2011) Nat Rev Immunol 11, 723-37.
  7. Rabinowitz, S.S. and Gordon, S. (1991) J Exp Med 174, 827-36.
  8. Holness, C.L. and Simmons, D.L. (1993) Blood 81, 1607-13.
  9. Ramprasad, M.P. et al. (1995) Proc Natl Acad Sci U S A 92, 9580-4.
  10. Kohrgruber, N. et al. (1999) J Immunol 163, 3250-9.
  11. Qualai, J. et al. (2016) PLoS One 11, e0154253.
  12. Tedder, T.F. et al. (1997) Immunity 6, 107-18.
  13. Scheuermann, R.H. and Racila, E. (1995) Leuk Lymphoma 18, 385-97.
  14. Cunningham, B.A. et al. (1987) Science 236, 799-806.
  15. Robertson, M.J. and Ritz, J. (1990) Blood 76, 2421-38.
  16. Moll, R. et al. (1982) Cell 31, 11-24.
  17. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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