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84223
Human Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Human Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit #84223

Citations (0)
Immunohistochemical analysis of paraffin-embedded normal cynomolgus monkey spleen using CD68 (D4B9C) XP® Rabbit mAb.
Simple Western™ analysis of lysates (0.1 mg/mL) from serum-starved HeLa cells treated with IFN-alpha (100 ng/mL, 5 min) using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Immunoprecipitation of Phospho-Stat1 (Tyr701) from HeLa cell extracts, treated with treated with IFNa (#36000, 100 ng/mL, 5 min). Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb, #9167.
Western blot analysis of extracts from THP-1, Raji, or Jurkat cells using CD11c (D3V1E) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Immunohistochemical analysis of paraffin-embedded human tonsil using CD68 (D4B9C) XP® Rabbit mAb.
Western blot analysis of extracts from HeLa cells, untreated or treated with IFNa (#36000, 100 ng/mL, 5 min) or IFNg (#80385, 100 ng/mL, 30 min); using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 (upper), Stat1 (D1K9Y) Rabbit mAb #14994 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Flow cytometric analysis of U266B1 cells, untreated (blue) or treated with hIFN-β (100 ng/ml, 5 mins; green) using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412.
Western blot analysis of extracts from various cell lines using CD86 (E2G8P) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various cell lines using CD206/MRC1 (E2L9N) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from SU-DHL-1 and A-375 cells using CD163 (D6U1J) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower)
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected (+) with constructs expressing full-length human Myc/DDK-tagged HLA-DRA (hHLA-DRA-Myc/DDK), HLA-DPA1 (hHLA-DPA1-Myc/DDK), HLA-DOA (hHLA-DOA-Myc/DDK), HLA-DQA1 (hHLA-DQA1-MycDDK), HLA-DQA2 (hHLA-DQA2-Myc/DKK), or HLA-DMA (hHLA-DMA-Myc/DKK), using HLA-DRA (E9R2Q) XP® Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using CD11c (D3V1E) XP® Rabbit mAb performed on the Leica® BOND Rx.
Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using CD68 (D4B9C) XP® Rabbit mAb.
Western blot analysis of extracts from various cell lines, untreated or treated with EGF (100 ng/mL, 30 min), IFNa (100 ng/mL, 5 min), or PDGF (100 ng/mL, 5 min); using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using CD86 (E2G8P) Rabbit mAb performed on the Leica® BOND Rx.
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using CD206/MRC1 (E2L9N) Rabbit mAb performed on the Leica® BOND Rx.
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using CD163 (D6U1J) Rabbit mAb performed on the Leica® Bond Rx.
Western blot analysis of extracts from various cell lines using HLA-DRA (E9R2Q) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunohistochemical analysis of paraffin-embedded THP-1 cell pellet (left, positive) or Raji cell pellet (right, negative) using CD11c (D3V1E) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CD68 (D4B9C) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded Non-Hodgkin lymphoma control (left) or λ phosphatase treated (right), using Phospho-Stat1 (tyr701) (58D6) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma using CD86 (E2G8P) Rabbit mAb performed on the Leica® BOND Rx.
Immunohistochemical analysis of paraffin-embedded MUTZ-3 cell pellet (left, positive) or THP-1 cell pellet (right, negative) using CD206/MRC1 (E2L9N) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human clear cell adenocarcinoma of the ovary using CD163 (D6U1J) Rabbit mAb performed on the Leica® Bond Rx.
Immunohistochemical analysis of paraffin-embedded human bladder adenocarcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb performed on the Leica® BOND Rx.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD11c (D3V1E) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded Non-Hodgkin lymphoma using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 in the presence of control peptide (left) or Phospho-Stat1 (Tyr701) Blocking Peptide (right).
Immunohistochemical analysis of paraffin-embedded human tonsil using CD86 (E2G8P) Rabbit mAb performed on the Leica® BOND Rx. 
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD206/MRC1 (E2L9N) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD163 (D6U1J) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb performed on the Leica® BOND Rx.
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using CD11c (D3V1E) XP® Rabbit mAb.
Multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using CD68 (D4B9C) XP® rabbit mAb (red), PD-1 (EH33) mouse mAb #43248 (green), CD8α (C8/144B) mouse mAb #70306 (magenta), Pan-keratin (C11) mouse mAb #4545 (cyan), LAG3 (D2G4O) XP® rabbit mAb #15372 (orange), and TIM-3 (D5D5R) XP® rabbit mAb #45208 (yellow).
Immunohistochemical analysis of paraffin-embedded stomach (chronic gastritis), using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using CD86 (E2G8P) Rabbit mAb performed on the Leica® BOND Rx.
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using CD206/MRC1 (E2L9N) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using CD163 (D6U1J) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb performed on the Leica® BOND Rx.
Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using CD11c (D3V1E) XP® Rabbit mAb.
Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using CD68 (D4B9C) XP® rabbit mAb (orange), PD-L1 (E1L3N®) XP® rabbit mAb #13684 (red), PD-L2 (D7U8C) XP® rabbit mAb (magenta) #82723, Arginase-1 (D4E3M) XP® rabbit mAb #93668 (green), IDO (D5J4E) rabbit mAb #86630 (yellow), and Pan-keratin (C11) mouse mAb #4545 (cyan).
Immunohistochemical analysis using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb on SignalSlide® HeLa -/+ IFNa IHC Controls #55861 (paraffin-embedded HeLa cell pellets, untreated (left) or treated with Human Interferon-α1 (hIFN-α1) #8927 (right)).
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using CD86 (E2G8P) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using CD206/MRC1 (E2L9N) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using CD163 (D6U1J) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded Raji cell pellet (left, positive) or Jurkat cell pellet (right, negative) using HLA-DRA (E9R2Q) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded THP-1 (left) and Jurkat (right) cell pellets using CD68 (D4B9C) XP® Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or IFNα-treated #9906 (right), using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of human MUTZ-3 cells (left, positive) and Jurkat cells (right, negative) using CD206/MRC1 (E2L9N) Rabbit mAb #91992 (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunohistochemical analysis of paraffin-embedded human serous carcinoma of the ovary using CD163 (D6U1J) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human mucoepidermoid carcinoma of the larynx using HLA-DRA (E9R2Q) XP® Rabbit mAb.
Confocal immunofluorescent analysis of THP-1 cells, differentiated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (80 nM, 24 hr) followed by 24 hr without TPA, (left, positive) or HeLa cells (right, negative), using CD11c (D3V1E) XP® Rabbit mAb #45581 (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Confocal immunofluorescent analysis of THP-1 (left) and Jurkat (right) cells using CD68 (D4B9C) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Projected images from a z-stack series are shown.
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with IFN-α (100ng/ml, 5 mins; green) using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412.
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using CD86 (E2G8P) Rabbit mAb (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemical analysis of paraffin-embedded normal rhesus monkey spleen using CD163 (D6U1J) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Flow cytometric analysis of live human peripheral blood mononuclear cells co-stained with anti-human CD14 using CD68 (D4B9C) XP® Rabbit mAb (right) compared to a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Immunohistochemical analysis of paraffin-embedded HDLM-2 cell pellet (left, positive) or HT-29 cell pellet (right, negative) using CD86 (E2G8P) Rabbit mAb.
Confocal immunofluorescent analysis of HDLM-2 cells (left, positive) and HT-29 cells (right, negative) using CD86 (E2G8P) Rabbit mAb (green). Blue = Hoechst 33342 #4082 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded SU-DHL-1 cell pellet (left, positive) or A-375 cell pellet (right, negative) using CD163 (D6U1J) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human T-cell lymphoma using HLA-DRA (E9R2Q) XP® Rabbit mAb.
Flow cytometric analysis of fixed and permeabilized human peripheral blood mononuclear cells co-stained with anti-human CD14 using CD68 (D4B9C) XP® Rabbit mAb (right) compared to a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Chromatin immunoprecipitations were performed with cross-linked chromatin from HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes and Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across IRF-1, a known target gene of Phospho-Stat1 (see additional figure containing ChIP-qPCR data).
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb.
Flow cytometric analysis of fixed and permeabilized Jurkat cells (blue, negative) and THP-1 cells (green, positive) using CD68 (D4B9C) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Chromatin immunoprecipitations were performed with cross-linked chromatin from HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes and Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across chromosome 5 (upper), including IRF-1 (lower), a known target gene of Phospho-Stat1 (see additional figure containing ChIP-qPCR data).
Immunohistochemical analysis of paraffin-embedded ovarian carcinoma (left) or colon carcinoma (right) using HLA-DRA (E9R2Q) XP® Rabbit mAb (top) or HLA-DRA Rabbit mAb (bottom). These two antibodies detect independent, unique epitopes on human HLA-DRA. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Chromatin immunoprecipitations were performed with cross-linked chromatin from HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes and either Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human TAP1 Promoter Primers #5148, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb.
Western blot analysis of extracts from HeLa cells untreated or treated with interferon-α (IFN-α), using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (upper) or Stat1 Antibody (#9172) (lower).
To Purchase # 84223
Cat. # Size Qty. Price
84223T
1 Kit  (6 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CD68 (D4B9C) XP® Rabbit mAb 76437 20 µl
  • IHC
  • IF
  • F
H Mk Rabbit IgG
CD163 (D6U1J) Rabbit mAb 93498 20 µl
  • WB
  • IHC
H Mk 160, 170 Rabbit IgG
CD206/MRC1 (E2L9N) Rabbit mAb 91992 20 µl
  • WB
  • IHC
  • IF
H 190-250 Rabbit IgG
CD11c (D3V1E) XP® Rabbit mAb 45581 20 µl
  • WB
  • IHC
  • IF
H 145 Rabbit IgG
CD86 (E2G8P) Rabbit mAb 91882 20 µl
  • WB
  • IHC
  • IF
H Mk 60-85 Rabbit IgG
HLA-DRA (E9R2Q) XP® Rabbit mAb 97971 20 µl
  • WB
  • IHC
H 30-40 Rabbit IgG
Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb 9167 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
  • ChIP
H M 84, 91 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Human Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit provides an economical means of characterizing the extent of M1 and M2 macrophages in formalin-fixed, paraffin-embedded tissue samples.

Specificity / Sensitivity

Each antibody in the Human Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit detects endogenous levels of its target human protein. CD11c (D3V1E) XP® Rabbit mAb does not cross-react with CD11b. Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb detects endogenous levels of Stat1 only when phosphorylated at tyrosine 701. The antibody detects phosphorylated tyrosine 701 of p91 Stat1 and also the p84 splice variant. It does not cross-react with the corresponding phospho-tyrosines of other Stat proteins.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a recombinant protein specific to human CD68, CD163, CD206/MRC1, and CD11c, or with synthetic peptides corresponding to residues surrounding Val153 of human HLA-DRA protein and Pro239 of human CD86 protein, or with synthetic phosphopeptides corresponding to residues surrounding Tyr701 of human Stat1 protein.

Background

Macrophages are myeloid cells of the innate immune system that are found in all human tissues in the body and exhibit anatomical and functional diversity. These heterogenous cells are derived from monocyte precursors in the blood that infiltrate into the tissues and differentiate in the presence of cytokines and growth factors. A spectrum of different macrophage phenotypes, or polarizations, have been described based on their secretory profiles, gene expression, and functions. Macrophages have great plasticity and can switch from one phenotype to another under different conditions. At the opposite extremes of this spectrum are so called M1, or classically activated phenotype, and M2 or alternatively activated phenotype. M1 macrophages are generally inflammatory and anti-tumor, while M2 macrophages, commonly referred to as tumor-associated macrophages (TAMs), are generally anti-inflammatory and pro-tumor. Relative contents of M1 and M2 macrophages in the tumor microenvironment may have prognostic values. Modulating macrophage polarization is actively pursued as a therapeutic intervention for many different diseases (1-6).
In humans, CD68 is considered a general marker for macrophages. CD11c, CD86, HLA-DRA, phospho-STAT1 (Tyr701), and others have been used as markers for M1 macrophages, while CD163, CD206, and others have been used as markers for M2 macrophages (7-10).

Pathways

Explore pathways related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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