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44689
Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit #44689

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Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 1

Immunohistochemical analysis of paraffin-embedded human infiltrating papillary carcinoma of the breast using PD-1 (D4W2J) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 2

Flow cytometric analysis of fixed and permeabilized human peripheral blood mononuclear cells, untreated (left column) or treated with anti-CD3 (10ug/ml, 72hr) and anti-CD28 (5ug/ml, 72 hr; right column), using PD-1 (D4W2J) XP® Rabbit mAb #86163 (top row) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (bottom row), and co-stained with CD3 (UCHT1) Mouse mAb (APC Conjugate) #19881.  Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 3

Flow cytometric analysis of Jurkat cells (blue) and primary CD4+ T cells (green) using TIM-3 (D5D5R™) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 4

Western blot analysis of extracts from primary human CD4+ T cells and various cell lines using TIM-3 (D5D5R™) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 5

Immunohistochemical analysis of paraffin-embedded renal clear cell carcinoma using TIM-3 (D5D5R™) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 6

Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma using LAG3 (D2G4O™) XP® Rabbit mAb performed on the Leica® Bond™ Rx.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 7

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using VISTA (D1L2G™) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 8

Flow cytometric analysis of fixed and permeabilized human whole blood cells stained with VISTA (D1L2G™) XP® Rabbit mAb or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 and co-stained with CD19-PE and CD14-APC. VISTA is present on CD14+ monocytes (right; upper right quadrant) and shows a significant increase in intensity when compared to its isotype control (middle; upper left quadrant). CD19+ B cells are negative for VISTA (left; upper left quadrant). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 9

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using B7-H3 (D9M2L) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 10

Flow cytometric analysis of human peripheral blood mononuclear cells (green), treated with anti-CD3 (10 μg/ml, 72h) and anti-CD28 (5 μg/ml, 72h), using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (dashed lines) co-stained with a CD8 antibody. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Analysis was performed on CD8+ cells.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 11

Western blot analysis of extracts from various endogenous human and mouse cells using OX40 (E9U7O) XP® Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). CD4+ T cells were purified from human blood or mouse spleens, and stimulated for 9 days using beads coated with human or mouse CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (20 ng/ml) or Mouse Interleukin-2 (mIL-2) #5201 (20 ng/ml).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 12

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using GITR (D9I9D) Rabbit mAb (IHC Preferred) performed on the Leica® BOND™ Rx.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 13

Immunohistochemical analysis of paraffin-embedded human T-cell non-Hodgkin's lymphoma using CD40 Ligand (D5J9Y) Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 14

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PD-1 (D4W2J) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 15

Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing full-length human Myc/DDK-tagged TIM-3 (hTIM-3-Myc/DDK; +), using TIM-3 (D5D5R™) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 16

Immunohistochemical analysis of paraffin-embedded human tonsil using TIM-3 (D5D5R™) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 17

Immunohistochemical analysis of paraffin-embedded HDLM-2 (left) and PC-3 (right) cell pellets on SignalSlide® PD-L1 IHC Controls #13747 using LAG3 (D2G4O™) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 18

Immunohistochemical analysis of paraffin-embedded human tonsil using VISTA (D1L2G™) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 19

Immunohistochemical analysis of paraffin-embedded lung carcinoma using B7-H3 (D9M2L) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 20

Flow cytometric analysis of Jurkat cells (blue) and HDLM-2 cells (green), using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 21

Immunohistochemical analysis of paraffin-embedded human papillary carcinoma of the breast using OX40 (E9U7O) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 22

Immunohistochemical analysis of paraffin-embedded human tonsil using OX40 (E9U7O) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 23

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using GITR (D9I9D) Rabbit mAb (IHC Preferred).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 24

Immunohistochemical analysis of paraffin-embedded Jurkat (left) and Raji (right) cell pellets using CD40 Ligand (D5J9Y) Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 25

Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin's lymphoma using PD-1 (D4W2J) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 26

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using TIM-3 (D5D5R™) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 27

Immunohistochemical analysis of paraffin-embedded human colitis using LAG3 (D2G4O™) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 28

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's B-Cell lymphoma using VISTA (D1L2G™) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 29

Immunohistochemical analysis of paraffin-embedded colon carcinoma using B7-H3 (D9M2L) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 30

Confocal immunofluorescent analysis of primary human CD8+ T cells treated with anti-CD3 and anti-CD28 in the presence of human interleukin-2 (left, positive) and HeLa cells (right, negative) using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 31

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using OX40 (E9U7O) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 32

Immunohistochemical analysis of paraffin-embedded human HuT 102 (left) and Jurkat (right) cell pellets using GITR (D9I9D) Rabbit mAb (IHC Preferred).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 33

Immunoprecipitation of CD40 ligand from a HeLa cell lysate spiked with recombinant Human CD40 Ligand (hCD40L) #3583 (10 ng/ml) using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CD40 Ligand (D5J9Y) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using CD40 Ligand (D5J9Y) Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 34

Immunohistochemical analysis of paraffin-embedded 293 cell pellets, control (left) or PD-1 transfected (right), using PD-1 (D4W2J) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 35

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TIM-3 (D5D5R™) XP® Rabbit mAb. Note staining of alveolar macrophages.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 36

Immunohistochemical analysis of paraffin-embedded human tonsil using LAG3 (D2G4O™) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 37

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using VISTA (D1L2G™) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 38

Immunohistochemical analysis of paraffin-embedded ovarian carcinoma using B7-H3 (D9M2L) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 39

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 40

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using OX40 (E9U7O) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 41

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using GITR (D9I9D) Rabbit mAb (IHC Preferred).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 42

Immunoprecipitation of CD40 ligand from Jurkat cells using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CD40 Ligand (D5J9Y) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using CD40 Ligand (D5J9Y) Rabbit mAb. A conformation specific secondary antibody was used to avoid reactivity with IgG heavy and light chains.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 43

Multiplex immunohistochemical analysis of paraffin-embedded human breast carcinoma usng PD-1 (D4W2J) XP® rabbit mAb (green), PD-L1 (E1L3N®) XP® rabbit mAb #13684 (red), LAG3 (D2G4O™) XP® rabbit mAb #15372 (magenta), TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow), CD8α (C8/144B) mouse mAb #70306 (orange), and Pan-keratin (C11) mouse mAb #4545 (cyan).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 44

Immunohistochemical analysis of paraffin-embedded cell pellets, primary CD4+ T cells (left) and HT-29 cells (right), using TIM-3 (D5D5R™) XP® Rabbit mAb. CD4+ T cells were purified from human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 45

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using VISTA (D1L2G™) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 46

Immunohistochemical analysis of paraffin-embedded LNCaP (left) and NCI-H69 (right) cell pellets using B7-H3 (D9M2L) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 47

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 48

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using OX40 (E9U7O) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 49

Western blot analysis of extracts from human CD8+ T cells, HuT 102, and Jurkat cells using GITR (D9I9D) Rabbit mAb (IHC Preferred) (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower). CD8+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of human interleukin-2 (hIL-2) #8907 (6.7 ng/ml).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 50

Western blot analysis of extracts from various cell lines and tissues using CD40 Ligand (D5J9Y) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 51

Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using PD-1 (D4W2J) XP® rabbit mAb (green), B7-H3 (D9M2L) XP® rabbit mAb #14058 (red), B7-H4 (D1M8I) XP® rabbit mAb (cyan), LAG3 (D2G4O™) XP® rabbit mAb #15372 (magenta), TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow), and VISTA (D1L2G™) XP® rabbit mAb #64953 (orange).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 52

Immunohistochemical analysis of paraffin-embedded OVKATE (left) and MCF7 (right) cell pellets using VISTA (D1L2G™) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 53

Immunohistochemical analysis of paraffin-embedded HDLM-2 cell pellet (left, positive) or HT-29 cell pellet (right, negative) using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 54

Immunohistochemical analysis of paraffin-embedded HuT 78 cell pellet (left, positive) or Jurkat cell pellet (right, negative) using OX40 (E9U7O) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 55

Western blot analysis of indicated amounts of recombinant Human CD40 Ligand (hCD40L) #3583 using CD40 Ligand (D5J9Y) Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 56

Multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using TIM-3 (D5D5R™) XP® rabbit mAb, PD-1 (EH33) mouse mAb #43248 (green), CD8α (C8/144B) mouse mAb #70306 (magenta), CD68 (D4B9C) XP® rabbit mAb #76437 (red), Pan-keratin (C11) mouse mAb #4545 (cyan),and LAG3 (D2G4O™) XP® rabbit mAb #15372 (orange).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 57

Western blot analysis of extracts from various cell lines using B7-H3 (D9M2L) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 58

Immunohistochemical analysis of paraffin-embedded human thymus using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 59

Immunohistochemical analysis of paraffin-embedded mouse spleen using OX40 (E9U7O) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 60

Immunoprecipitation of PD-1 protein from Molt-4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PD-1 (D4W2J) XP® Rabbit mAb. Western blot analysis was performed using PD-1

(D4W2J) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 61

Western blot analysis of extracts from HDLM-2, HuT 78, and Jurkat cells using LAG3 (D2G4O™) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 62

Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with constructs expressing Myc-tagged full-length human PD-L1, PD-L2, B7-H3, or B7-H4 protein (as indicated), using B7-H3 (D9M2L) XP® Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 63

Immunoprecipitation of 4-1BB/CD137/TNFRSF9 from HDLM-2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb. Western blot analysis was performed using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 64

Immunohistochemical analysis of paraffin-embedded rat thymus using OX40 (E9U7O) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 65

Western blot analysis of extracts from human CD4+ T cells, MOLT-4, and Jurkat cells using PD-1 (D4W2J) XP® Rabbit mAb (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower). CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of human interleukin-2 (hIL-2) #8907 (6.7 ng/ml).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 66

Western blot analysis of extracts from OVKATE, DU 145, and MCF7 cells using VISTA ((D1L2G™) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 67

Western blot analysis of extracts from primary human CD8+ T cells, treated with anti-CD3 and anti-CD28 in the presence of human interleukin-2, and various cell lines using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 68

Immunoprecipitation of OX40 protein from HuT 102 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is OX40 (E9U7O) XP® Rabbit mAb. Western blot analysis was performed using OX40 (E9U7O) XP® Rabbit mAb.

Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit: Image 69

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human OX40 (Myc/DDK Human OX40; +) or mouse OX40 (Myc/DDK Mouse OX40; +), using OX40 (E9U7O) XP® Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 44689T
Product # Size Price
44689T
1 Kit  (9 x 20 µl) $ 582

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
PD-1 (D4W2J) XP® Rabbit mAb 86163 20 µl
  • WB
  • IP
  • IHC
  • F
H 52-65 Rabbit 
TIM-3 (D5D5R™) XP® Rabbit mAb 45208 20 µl
  • WB
  • IP
  • IHC
  • F
H 45-70 Rabbit IgG
LAG3 (D2G4O™) XP® Rabbit mAb 15372 20 µl
  • WB
  • IHC
H 60-80 Rabbit IgG
VISTA (D1L2G™) XP® Rabbit mAb 64953 20 µl
  • WB
  • IHC
  • F
H Mk 45-65 Rabbit IgG
B7-H3 (D9M2L) XP® Rabbit mAb 14058 20 µl
  • WB
  • IHC
H 90 Rabbit IgG
4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb 34594 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H 32, 40 Rabbit IgG
OX40 (E9U7O) XP® Rabbit mAb 61637 20 µl
  • WB
  • IP
  • IHC
H M R 35-50 Rabbit IgG
GITR (D9I9D) Rabbit mAb (IHC Preferred) 68014 20 µl
  • WB
  • IHC
H 25 Rabbit IgG
CD40 Ligand (D5J9Y) Rabbit mAb 15094 20 µl
  • WB
  • IP
  • IHC
H 25-30 (membrane bound); 17 (soluble) Rabbit IgG

Product Description

The Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit provides an economical means of detecting expression of receptors that modulate T cell activity in formalin-fixed, paraffin-embedded tissue samples.

Specificity / Sensitivity

Each antibody included in the Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC Antibody Sampler Kit recognizes endogenous levels of its target protein. CD40 Ligand (D5J9Y) Rabbit mAb recognizes endogenous levels of total membrane bound and soluble CD40 ligand protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala274 of human PD-1 protein, Gln264 of human OX40 protein, Val142 of human GITR protein, Ala94 of human B7-H3 protein, or near the carboxy terminus of human VISTA protein. Monoclonal antibodies are produced by immunizing animals with a recombinant protein specific to the extracellular domain of human TIM-3 protein, human 4-1BB/CD137/TNFRSF9 protein, human CD40 ligand protein, or the amino terminus of human LAG3 protein.

Background

PD-1 (PDCD1, CD279), TIM-3 (HAVCR2), LAG3 (CD223), VISTA (PD-H1), and B7-H3 (CD276) are immune cell co-inhibitory receptors (also known as immune checkpoints) that negatively regulate T cell function, and dampen the immune response to pathogens and cancer. In addition to activated T cells, PD-1 is expressed by activated B-cells and monocytes. TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer. Tumor-infiltrating macrophages and dendritic cells also express TIM-3. LAG3 is primarily expressed by activated CD4+ T cells, CD8+ T cells, FoxP3+ T regulatory cells (Tregs) and natural killer (NK) cells. Although primarily expressed by myeloid cells, VISTA is also expressed by CD4+, CD8+, and Treg cells. Research examining the biological function of B7-H3 suggested that B7-H3 can be both a positive and negative regulator of T cell response. B7-H3 is expressed by antigen presenting cells, activated T cells, and a few normal tissues, including placenta and prostate. Expression of B7-H3 is seen in several cancer types, including prostate, breast, colon, lung, and gastric cancers, and in endothelial cells from tumor associated vasculature. Therapeutic blockade of these immune checkpoint receptors is a promising strategy for neoplastic intervention by enabling anti-tumor immune responses (1-3).

4-1BB (TNFRSF9, CD137), GITR (TNFRSF18), OX40 (TNFRSF4, CD134), and CD40 ligand (CD40L, CD154, TRAP, gp39) are immune cell co-stimulatory receptors that promote effector T cell survival and activation, and enable optimal immune responses to pathogens. 4-1BB is expressed in activated CD4+ and CD8+ T cells, natural killer cells and dendritic cells. GITR is expressed constitutively at high levels on Tregs, at low levels on naive and memory T cells, and is induced upon T cell activation. Studies show GITR can also be induced on NK cells, macrophages, and DCs. GITR ligation has been shown to induce CD8+ T cell activation, cytoxicity, and memory T cell survival, and conversely inhibit Treg suppressive function while promoting effector T cell resistance to Treg suppression. OX40 is primarily expressed on activated CD4+ and CD8+ T cells, while CD40L is primarily expressed on the surface of T cells, but has also been reported in blood platelets, mast cells, basophils, NK cells, and B cells. Research studies show that agonists of these co-stimulatory receptors augment anti-tumor immunity in several cancer types. Due to the combined effects on both Treg suppression and effector cell activation, GITR represents a unique opportunity for immunotherapeutic intervention in cancer. These pathways are an important area of interest in the study of cancer, vascular diseases, and inflammatory disorders (4-7).

  1. Schildberg, F.A. et al. (2016) Immunity 44, 955-72.
  2. Anderson, A.C. et al. (2016) Immunity 44, 989-1004.
  3. Callahan, M.K. et al. (2016) Immunity 44, 1069-78.
  4. Ward-Kavanagh, L.K. et al. (2016) Immunity 44, 1005-19.
  5. Ara, A. et al. (2018) Immunotargets Ther 7, 55-61.
  6. Knee, D.A. et al. (2016) Eur J Cancer 67, 1-10.
  7. Chester, C. et al. (2018) Blood 131, 49-57.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
D1L2G is a trademark of Cell Signaling Technology. Inc.
D2G4O is a trademark of Cell Signaling Technology. Inc.
D5D5R is a trademark of Cell Signaling Technology. Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
BOND is a trademark of Leica Biosystems Melbourne Pty. Ltd. No affiliation or sponsorship between CST and Leica Microsystems IR GmbH or Leica Biosystems Melbourne Pty. Ltd is implied.
LEICA is a registered trade​mark of Leica Microsystems IR GmbH.
Tween is a registered trademark of ICI Americas, Inc.