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9770
IAP Family Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

IAP Family Antibody Sampler Kit #9770

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IAP Family Antibody Sampler Kit: Image 1

Western blot analysis of extracts from various cell lines using c-IAP1 (D5G9) Rabbit mAb.

IAP Family Antibody Sampler Kit: Image 2

Western blot analysis of extracts from Raji, Ramos and SR cell lines, using c-IAP2 (58C7) Rabbit mAb.

IAP Family Antibody Sampler Kit: Image 3

Flow cytometric analysis of untreated Jurkat cells using Survivin (71G4B7E) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

IAP Family Antibody Sampler Kit: Image 4

Western blot analysis of extracts from HeLa, Raji and COS cell lines, using XIAP (3B6) Rabbit mAb.

IAP Family Antibody Sampler Kit: Image 5

Confocal immunofluorescent analysis of SK-MEL-28 cells (left) and HeLa cells (right) using Livin (D61D1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

IAP Family Antibody Sampler Kit: Image 6

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

IAP Family Antibody Sampler Kit: Image 7

Western blot analysis of extracts from HeLa cells, mock transfected (-) or transfected with human c-IAP1 (+), using c-IAP1 (D5G9) Rabbit mAb.

IAP Family Antibody Sampler Kit: Image 8

Confocal immunofluorescent analysis of HeLa cells using Survivin (71G4B7E) Rabbit mAb (green). Mitochondria have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IAP Family Antibody Sampler Kit: Image 9

Western blot analysis of extracts from various cell lines using Livin (D61D1) XP® Rabbit mAb.

IAP Family Antibody Sampler Kit: Image 10

Immunohistochemical analysis of frozen H1650 xenograft showing nuclear localization using Survivin (71G4B7E) Rabbit mAb.

IAP Family Antibody Sampler Kit: Image 11

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder using Survivin (71G4B7E) Rabbit mAb.

IAP Family Antibody Sampler Kit: Image 12

Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing nuclear localization using Survivin (71G4B7E) Rabbit mAb #2808.

IAP Family Antibody Sampler Kit: Image 13

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Survivin (71G4B7E) Rabbit mAb in the presence of control peptide (left) or Survivin Blocking Peptide #1037 (right).

IAP Family Antibody Sampler Kit: Image 14

Immunohistochemical analysis of paraffin-embedded human pituitary adenoma using Survivin (71G4B7E) Rabbit mAb.

IAP Family Antibody Sampler Kit: Image 15

Western blot analysis of lysates from Raji (human), BaF3 (mouse) and C6 (rat) cell lines using Survivin (71G4B7E) Rabbit mAb.

IAP Family Antibody Sampler Kit: Image 16

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® Survivin siRNA II (+), using Survivin (71G4B7E) Rabbit mAb #2808 and α-Tubulin (11H10) Rabbit mAb #2125. Survivin (71G4B7E) Rabbit mAb confirms silencing of survivin expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of survivin siRNA.

To Purchase # 9770T
Product # Size Price
9770T
1 Kit  (5 x 20 µl) $ 391

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
c-IAP1 (D5G9) Rabbit mAb 7065 20 µl
  • WB
  • IP
H 62 Rabbit IgG
c-IAP2 (58C7) Rabbit mAb 3130 20 µl
  • WB
  • IP
H 70 Rabbit IgG
Survivin (71G4B7) Rabbit mAb 2808 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R 16 Rabbit IgG
XIAP (3B6) Rabbit mAb 2045 20 µl
  • WB
H Mk 53 Rabbit IgG
Livin (D61D1) XP® Rabbit mAb 5471 20 µl
  • WB
  • IP
  • IF
H 34, 36 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The IAP Family Antibody Sampler Kit provides an economical means to investigate the expression of various IAP family members within the cell. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.

Specificity / Sensitivity

Each antibody in the IAP Family Antibody Sampler Kit detects endogenous levels of its respective target.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu28 of human c-IAP1 protein. The antibody is purified by protein A and affinity chromatography. Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Val117 of human c-IAP2, Cys60 of human Survivin, Ser245 of human XIAP, and Ala195 of human Livin.

Background

The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2).

  1. Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52.
  2. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
  3. Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33.
  4. Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803.
  5. Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46.
  6. Deveraux, Q.L. et al. (1997) Nature 388, 300-4.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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