WB, IHC-P
H Mk
Endogenous
46
Rat IgG2a
#O75874
3417
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to human IDH1 protein (9,10).
Background
IDH1 is one of three isocitrate dehydrogenases that catalyze the oxidative decarboxylation of isocitrate to α-ketoglutarate (α-KG). These enzymes exist in two distinct subclasses that utilize either NAD or NADP+ respectively, as an electron acceptor (1). IDH1 is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. IDH2 and 3 are mitochondrial enzymes that also function in the Krebs cycle. IDH1 is inactivated by phosphorylation at Ser113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock (2,3). IDH1 is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle. Mutations in IDH1 have been reported in glioblastoma (4), acute myeloid leukemia (5,6), and other malignancies (7). IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway (8).
- Ramachandran, N. and Colman, R.F. (1980) J Biol Chem 255, 8859-64.
- Bennett, P.M. and Holms, W.H. (1975) J Gen Microbiol 87, 37-51.
- Hurley, J.H. et al. (1990) Science 249, 1012-6.
- Bleeker, F.E. et al. (2009) Hum Mutat 30, 7-11.
- Abbas, S. et al. (2010) Blood 116, 2122-6.
- Paschka, P. et al. (2010) J Clin Oncol 28, 3636-43.
- Watanabe, T. et al. (2009) Am J Pathol 174, 1149-53.
- Zhao, S. et al. (2009) Science 324, 261-5.
- Kaneko, M.K. et al. (2013) Monoclon Antib Immunodiagn Immunother 32, 224-8.
- Kato, Y. (2015) Brain Tumor Pathol 32, 3-11.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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