Revision 1
#3159
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP, E-P
Reactivity:
H
Sensitivity:
Recombinant protein
MW (kDa):
17
Source/Isotype:
Mouse IgG1
UniProt ID:
#P01579
Entrez-Gene Id:
3458
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Peptide ELISA (DELFIA) | 1:100 |
Storage
Specificity/Sensitivity
Source / Purification
Background
IFN-γ, also known as type II interferon, is produced mainly in activated T lymphocytes and natural killer cells (4) and has broad effects on various cells of the immune system. Many signaling proteins including IL-2, FGF, and EGF induce the synthesis of IFN-γ.
Background References
- Schroder, K. et al. (2004) J Leukoc Biol 75, 163-89.
- Martinez, F.O. et al. (2009) Annu Rev Immunol 27, 451-83.
- Kotenko, S.V. et al. (1995) J Biol Chem 270, 20915-21.
- McLaren, J.E. and Ramji, D.P. (2009) Cytokine Growth Factor Rev 20, 125-35.
- Kelker, H.C. et al. (1984) J Biol Chem 259, 4301-4.
- Young, H.A. and Hardy, K.J. (1995) J Leukoc Biol 58, 373-81.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation E-P: Peptide ELISA (DELFIA)
Cross-Reactivity Key
H: Human
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Limited Uses
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