WB
H M
Endogenous
78
Rabbit IgG
#Q9NPH3
3556
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human IL1RAP protein.
Background
Interleukin-1 receptor accessory protein (IL1RAP/IL1R3) is a type-I transmembrane glycoprotein and component of the IL-1 and IL-33 receptor signaling complexes (1,2). Research studies have demonstrated that IL1RAP expression is required for IL-1-mediated signaling through its ability to recruit IRAK to the IL-1R complex (3-7). In the context of human disease, research studies have shown that IL1RAP is overexpressed on the surface of AML stem cells and associates with c-KIT and FLT3 to mediate pro-proliferative and pro-survival signaling events downstream of these oncogenic receptor tyrosine kinases (8,9). As such, IL1RAP has emerged as a novel immuno-therapeutic target for AML and other malignancies of the myeloid compartment (10,11).
- Greenfeder, S.A. et al. (1995) J Biol Chem 270, 13757-65.
- Chackerian, A.A. et al. (2007) J Immunol 179, 2551-5.
- Radons, J. et al. (2002) J Biol Chem 277, 16456-63.
- Huang, J. et al. (1997) Proc Natl Acad Sci U S A 94, 12829-32.
- Wesche, H. et al. (1997) J Biol Chem 272, 7727-31.
- Gould, L. et al. (1979) J Clin Pharmacol 19, 46-55.
- Volpe, F. et al. (1997) FEBS Lett 419, 41-4.
- Mitchell, K. et al. (2018) J Exp Med 215, 1709-27.
- Barreyro, L. et al. (2012) Blood 120, 1290-8.
- Askmyr, M. et al. (2013) Blood 121, 3709-13.
- Ågerstam, H. et al. (2015) Proc Natl Acad Sci U S A 112, 10786-91.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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