Revision 1

#43069

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
200, 140

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q9NZM3

Entrez-Gene Id:
50618

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

Intersectin-2 (E5J9F) Rabbit Monoclonal Antibody recognizes endogenous levels of total intersectin-2 protein. This antibody reacts with intersectin-2L and intersectin-2S protein isoforms but does not cross-react with the corresponding isoforms of intersectin-1 protein. This antibody cross-reacts with proteins of unknown identity between 80 kDa and 120 kDa in some rat cell extracts.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys335 of human intersectin-2 protein.

Background

Intersectin-2 is a ubiquitously expressed, modular adaptor protein that participates in clathrin-mediated endocytosis through interaction with multiple proteins involved in trafficking of proteins through the endocytic pathway. Like its paralog, intersectin-1, there are two major isoforms of mammalian intersectin-2. Intersectin-2L and intersectin-2S are generated through alternative splicing of mRNA transcripts. The structure of both isoforms is characterized by two N-terminal EH domains, a central coiled-coil domain, followed by five tandem SH3 domains. The longer isoform, intersection-2L, contains an extended C-terminus with a Rho-GEF domain that controls the activity of the Cdc42 GTPase, a PH domain, and a calcium binding C2 domain (1-3). Research studies have shown that intersectin-2 plays a role in T cell receptor (TCR) signaling by promoting endocytosis of the TCR following its activation (4).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various human cell lines using Intersectin-2 (E5J9F) Rabbit mAb.
Western Blotting Image 1: Intersectin-2 (E5J9F) Rabbit Monoclonal Antibody
Western blot analysis of extracts from 293T cells, transfected with control siRNA (-) or Intersectin-2 siRNA (+), using Intersectin-2 (E5J9F) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 2: Intersectin-2 (E5J9F) Rabbit Monoclonal Antibody
Western blot analysis of extracts from various human and mouse cell lines using Intersectin-2 (E5J9F) Rabbit mAb.
Western Blotting Image 3: Intersectin-2 (E5J9F) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunoprecipitation of intersectin-2 protein from MOLT-4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Intersectin-2 (E5J9F) Rabbit mAb. Western blot analysis was performed using Intersectin-2 (E5J9F) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Immunoprecipitation Image 1: Intersectin-2 (E5J9F) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.