| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Phospho-IRS-1 (Ser302) (34C7) Rabbit mAb | 2491 | 20 µl | 180 kDa | Rabbit IgG |
| Phospho-IRS-1 (Ser307) Antibody | 2381 | 20 µl | 180 kDa | Rabbit |
| Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb | 5610 | 20 µl | 180 kDa | Rabbit IgG |
| Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb | 3203 | 20 µl | 180 kDa | Rabbit IgG |
| Phospho-IRS-1 (Ser636/639) Antibody | 2388 | 20 µl | 180 kDa | Rabbit |
| Phospho-IRS-1 (Ser1101) Antibody | 2385 | 20 µl | 180 kDa | Rabbit |
| IRS-1 (D23G12) Rabbit mAb | 3407 | 20 µl | 180 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The IRS-1 Inhibition Antibody Sampler Kit provides an economical means to evaluate insulin signaling negative feedback loops via phosphorylation of various IRS-1 serine residues. The kit includes enough antibody to perform two western blot experiments with each primary antibody.
Storage
Background
Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites, many of which are related to negative feedback loops activated during insulin signaling. Ser302 (human Ser307) of IRS-1 is regulated by FOX01 (5), IKKγ, and MYO1C (6). Ser307 (human Ser312) of IRS-1 is phosphorylated by JNK (7) and IKK (8). PKC phosphorylates mouse IRS-1 at Ser318 (human Ser323) by insulin receptor activation or by other stimulation such as TPA, IL-6, and retinoic acid treatment (9-12). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 (human Ser616) and Ser632/635 (human Ser636/639), respectively (13,14). Phosphorylation of IRS-1 at Ser1097 (human Ser1101) is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (15).
- Sun, X.J. et al. (1991) Nature 352, 73-7.
- Sun, X.J. et al. (1992) J Biol Chem 267, 22662-72.
- Myers, M.G. et al. (1993) Endocrinology 132, 1421-30.
- Wang, L.M. et al. (1993) Science 261, 1591-4.
- Cao, Y. et al. (2006) J Biol Chem 281, 40242-51.
- Nakamori, Y. et al. (2006) J Cell Biol 173, 665-71.
- Rui, L. et al. (2001) J Clin Invest 107, 181-9.
- Gao, Z. et al. (2002) J Biol Chem 277, 48115-21.
- Greene, M.W. et al. (2004) Biochem J 378, 105-16.
- Weigert, C. et al. (2006) J Biol Chem 281, 7060-7.
- del Rincón, S.V. et al. (2004) Oncogene 23, 9269-79.
- Moeschel, K. et al. (2004) J Biol Chem 279, 25157-63.
- Ozes, O.N. et al. (2001) Proc Natl Acad Sci U S A 98, 4640-5.
- De Fea, K. and Roth, R.A. (1997) Biochemistry 36, 12939-47.
- Li, Y. et al. (2004) J Biol Chem 279, 45304-7.
Background References
Trademarks and Patents
Limited Uses
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