Cat. # | Size | Qty. | Price |
---|---|---|---|
12879T | 1 Kit (7 x 20 microliters) |
|
Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
---|---|---|---|---|---|
Phospho-IRS-1 (Ser302) (34C7) Rabbit mAb 2491 | 20 µl |
|
H M | 180 | Rabbit IgG |
Phospho-IRS-1 (Ser307) Antibody 2381 | 20 µl |
|
H M R | 180 | Rabbit |
Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb 5610 | 20 µl |
|
H M | 180 | Rabbit IgG |
Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb 3203 | 20 µl |
|
H M R | 180 | Rabbit IgG |
Phospho-IRS-1 (Ser636/639) Antibody 2388 | 20 µl |
|
H M R | 180 | Rabbit |
Phospho-IRS-1 (Ser1101) Antibody 2385 | 20 µl |
|
H M R | 180 | Rabbit |
IRS-1 (D23G12) Rabbit mAb 3407 | 20 µl |
|
H M R Mk | 180 | Rabbit IgG |
Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Rab | Goat |
Product Information
Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding mouse Ser307 (human Ser312), human Ser636/639 (mouse Ser632/635), and human Ser1101 (mouse Ser1097) of IRS-1. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding mouse Ser302 (human Ser307), mouse Ser318 (human Ser323), and mouse Ser612 (human 616) of IRS-1. IRS-1 (D23G12) rabbit monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Ser270 of human IRS-1 (mouse 1097).
Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites, many of which are related to negative feedback loops activated during insulin signaling. Ser302 (human Ser307) of IRS-1 is regulated by FOX01 (5), IKKγ, and MYO1C (6). Ser307 (human Ser312) of IRS-1 is phosphorylated by JNK (7) and IKK (8). PKC phosphorylates mouse IRS-1 at Ser318 (human Ser323) by insulin receptor activation or by other stimulation such as TPA, IL-6, and retinoic acid treatment (9-12). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 (human Ser616) and Ser632/635 (human Ser636/639), respectively (13,14). Phosphorylation of IRS-1 at Ser1097 (human Ser1101) is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (15).
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