WB, IP, IF-IC
H
Endogenous
42, 84
Rabbit IgG
#Q9Y287
9445
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:3200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala105 of human ITM2B/Bri2 protein.
Background
Integral membrane protein 2b (ITM2B), also known as Bri2, is a type II membrane protein. ITM2B is expressed as a precursor immature form and is processed by furin proteases to produce the mature ITM2B/Bri2 protein and a soluble C-terminal fragment (1,2). The membrane-bound ITM2B/Bri2 protein can be further processed by ADAM10 and intramembrane proteases (1). Several studies have implicated the ITM2B gene with familial forms of dementia and neurodegenerative diseases, including Alzheimer’s disease (AD). Mutations in the human ITM2B gene are linked to several familial British and Danish dementia disorders (3,4). ITM2B gene mutations cause abnormal processing of the ITM2B/Bri2 protein, suggesting that the products of ITM2B/Bri2 protein cleavage might contribute directly to disease etiology (4). Interestingly, cleavage of disease-linked mutant ITM2B-encoded proteins generates peptides (ABri and ADan) that are more prone to deposit as amyloid fibrils, a pathological hallmark of many neurodegenerative diseases (2,4). Additionally, ITM2B/Bri2 interacts with Aβ-precursor protein (APP), a gene/protein linked to AD, and may alter APP processing and fibril formation (5-7). ITM2B/Bri2 is one of a family of proteins containing a BRICHOS domain, identified by their ability to inhibit Aβ fibril formation (8). The specific function of ITM2B/Bri2 is unclear, but it may contribute to normal synaptic function via an unknown mechanism (9).
- Tsachaki, M. et al. (2011) Glycobiology 21, 1382-8.
- Kim, S.H. et al. (1999) Nat Neurosci 2, 984-8.
- Vidal, R. et al. (1999) Nature 399, 776-81.
- Vidal, R. et al. (2000) Proc Natl Acad Sci U S A 97, 4920-5.
- Fotinopoulou, A. et al. (2005) J Biol Chem 280, 30768-72.
- Matsuda, S. et al. (2005) J Biol Chem 280, 28912-6.
- Matsuda, S. et al. (2008) J Neurosci 28, 8668-76.
- Matsuda, S. et al. (2011) Neurobiol Aging 32, 1400-8.
- Yao, W. et al. (2019) Sci Rep 9, 4862.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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