WB, IP, IF-IC
H
Endogenous
40
Rabbit IgG
#Q9Y624
50848
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central sequence of human JAM-A protein.
Background
Junctional Adhesion Molecule-A/F11 Receptor (JAM-A/F11R) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. JAM-A regulates multiple cellular processes, including tight junction assembly, epithelial-mesenchymal transition (EMT), leukocyte migration, virus binding, platelet activation, and angiogenesis. Aberrant expression of JAM-A is correlated with poor patient prognosis in several human cancers (1,2). In a mouse model of atherosclerosis, an antagonistic peptide that inhibits JAM-A-expressing platelets from interacting with inflamed endothelial cells reduces atherosclerotic plaque formation (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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