WB, IP, IF-F
H M R
Endogenous
130-260
Rabbit IgG
#Q9H2X9
57468
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Frozen) | 1:100 |
Storage
For a carrier free (BSA and azide free) version of this product see product #56440.
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala970 of human KCC2 protein.
Background
The potassium/chloride cotransporter 2 (KCC2, SLC12A5) is a neuron-specific transport protein responsible for regulating the cotransport of potassium and chloride ions. KCC2 uses the energy of the electrochemical potassium gradient to export chloride ions from cells, therefore maintaining intracellular chloride ion concentrations in mature neurons (1,2). The intracellular concentration of chloride ions determines the neuronal response to the inhibitory neurotransmitter GABA and glycine. As a result, KCC2 can play a critical role in regulating neuronal excitability in mature central nervous system neurons (3-5). Altered KCC2 expression and reduced KCC2 activity can result in an increase in intracellular chloride ion concentrations and subsequent hyperexcitability of neuronal systems. Cases of aberrant KCC2 function are associated with neurological disorders, such as multiple forms of epilepsy, neuropathic pain, and schizophrenia (6-10).
- Payne, J.A. et al. (1996) J Biol Chem 271, 16245-52.
- Delpire, E. (2000) News Physiol Sci 15, 309-12.
- Rivera, C. et al. (1999) Nature 397, 251-5.
- Woo, N.S. et al. (2002) Hippocampus 12, 258-68.
- Zhu, L. et al. (2005) J Neurophysiol 93, 1557-68.
- Arion, D. and Lewis, D.A. (2011) Arch Gen Psychiatry 68, 21-31.
- Hyde, T.M. et al. (2011) J Neurosci 31, 11088-95.
- Kaila, K. et al. (2014) Curr Opin Neurobiol 26, 34-41.
- Mòdol, L. et al. (2014) Pain 155, 1577-90.
- Kahle, K.T. et al. (2014) EMBO Rep 15, 766-74.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-F: Immunofluorescence (Frozen)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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